Danku John M C, Lahner Brett, Yakubova Elena, Salt David E
School of Biological Sciences, University of Aberdeen, Aberdeen, UK.
Methods Mol Biol. 2013;953:255-76. doi: 10.1007/978-1-62703-152-3_17.
Large-scale phenotyping methods are at the heart of efficiently deciphering the functions of genes and gene networks in the postgenomic era. In order to obtain meaningful results when comparing natural variants, and mutants and wild-types during large-scale quantitative analyses, necessary precautions must be employed throughout the whole process. Here, we describe large-scale elemental profiling in Arabidopsis thaliana and other genetic model organisms using high-throughput analytical methodologies. We also include a description of workflow management and data storage systems.
大规模表型分析方法是在后基因组时代有效解读基因和基因网络功能的核心。为了在大规模定量分析中比较自然变体、突变体和野生型时获得有意义的结果,在整个过程中必须采取必要的预防措施。在这里,我们描述了使用高通量分析方法对拟南芥和其他遗传模型生物进行大规模元素分析。我们还包括了工作流程管理和数据存储系统的描述。