Hirose T, Kudo E, Hasegawa T, Abe J, Hizawa K
First Department of Pathology, University of Tokushima School of Medicine, Japan.
Hum Pathol. 1990 Feb;21(2):204-11. doi: 10.1016/0046-8177(90)90130-w.
The immunohistochemical expression of cytoskeletal proteins in alveolar soft part sarcoma (ASPS) was studied by light and electron microscopy. Of the five cases examined by the avidinbiotin-peroxidase complex method, variable numbers of immunoreactive cells for desmin were found in three, for vimentin in two, for muscle-specific actins in three, and for alpha-smooth muscle actin in four. Immunoelectron microscopic study demonstrated that desmin and vimentin were localized on whorled bundles of intermediate filaments in the perinuclear cytoplasm. In addition, a few dispersed intermediate filaments became evident in specimens treated with saponin and fixed with tannic acid. These immunohistochemical results indicate that a few tumor cells of ASPS may express some properties of the cytoskeleton of smooth muscle cells in addition to those of skeletal muscle cells. Considering the discrepancies reported in the actin isoforms demonstrated in myogenic tumors, we conclude that ASPS is probably a peculiar, primitive myogenic tumor that does not show any distinctive features of rhabdomyogenic or leiomyogenic differentiation.
采用光镜和电镜研究了肺泡软组织肉瘤（ASPS）中细胞骨架蛋白的免疫组化表达。在采用抗生物素蛋白-生物素-过氧化物酶复合物法检测的5例病例中，3例发现有不同数量的结蛋白免疫反应性细胞，2例发现波形蛋白免疫反应性细胞，3例发现肌肉特异性肌动蛋白免疫反应性细胞，4例发现α-平滑肌肌动蛋白免疫反应性细胞。免疫电镜研究表明，结蛋白和波形蛋白定位于核周细胞质中呈涡状排列的中间丝束上。此外，在用皂苷处理并用单宁酸固定的标本中可见一些散在的中间丝。这些免疫组化结果表明，ASPS的一些肿瘤细胞除具有骨骼肌细胞的细胞骨架特性外，还可能表达平滑肌细胞的一些特性。考虑到在肌源性肿瘤中所显示的肌动蛋白同工型的差异，我们得出结论，ASPS可能是一种特殊的、原始的肌源性肿瘤，不表现出任何横纹肌源性或平滑肌源性分化的独特特征。
