Miettinen M, Lehto V P, Badley R A, Virtanen I
Int J Cancer. 1982 Nov 15;30(5):541-6. doi: 10.1002/ijc.2910300502.
Monospecific antibodies and indirect immunofluorescence microscopy were used to investigate the presence of cytoskeletal intermediate filaments of the keratin, vimentin and desmin types in 43 soft-tissue sarcomas. The results showed that vimentin was present in the neoplastic cells of all types of soft-tissue sarcomas studied, whereas keratin was absent, the only exception being the epithelial-like cells in biphasic synovial sarcoma. The presence of desmin was confined to leiomyosarcomas and rhabdomyosarcomas, which usually showed desminpositivity. In addition, malignant fibrous histiocytomas occasionally displayed some desmin-positive cells. Thus, determination of intermediate filaments is of aid in the histogenetical diagnosis of tumors, because keratin-positivity largely excludes the possibility of a sarcoma, and desmin-positivity aids in recognizing leiomyosarcomas and rhabdomyosarcomas.
采用单特异性抗体和间接免疫荧光显微镜技术，对43例软组织肉瘤中角蛋白、波形蛋白和结蛋白类型的细胞骨架中间丝的存在情况进行了研究。结果显示，在所研究的所有类型软组织肉瘤的肿瘤细胞中均存在波形蛋白，而角蛋白缺失，唯一的例外是双相滑膜肉瘤中的上皮样细胞。结蛋白仅存在于平滑肌肉瘤和横纹肌肉瘤中，这些肿瘤通常显示结蛋白阳性。此外，恶性纤维组织细胞瘤偶尔会出现一些结蛋白阳性细胞。因此，中间丝的检测有助于肿瘤的组织发生学诊断，因为角蛋白阳性很大程度上排除了肉瘤的可能性，而结蛋白阳性有助于识别平滑肌肉瘤和横纹肌肉瘤。
