DeJong J L, Mohandas T, Tu C P
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Genomics. 1990 Feb;6(2):379-82. doi: 10.1016/0888-7543(90)90580-n.
The microsomal glutathione S-transferase (GST) is a unique membrane-bound GST structurally distinct from the cytosolic GSTs. A cDNA encoding this 154 amino acid protein has recently been isolated and characterized. Using the cDNA as the hybridization probe, we now report the assignment of the human microsomal GST gene to chromosome 12 through the use of a panel of mouse-human somatic cell hybrid lines. This locus has recently been designated as GST 12. In addition, genomic Southern blotting data suggest that the human microsomal GST is encoded by a single- or very-low-copy gene. Therefore, the human GST gene superfamily resides on at least four separate chromosomes: 1 (GST 1), 6 (GST 2), 11 (GST 3), and 12 (GST 12).
微粒体谷胱甘肽S-转移酶（GST）是一种独特的膜结合型GST，其结构与胞质GST不同。最近已分离并鉴定出编码这种154个氨基酸蛋白质的cDNA。以该cDNA作为杂交探针，我们现在报告通过使用一组小鼠-人类体细胞杂交系将人类微粒体GST基因定位于12号染色体。该基因座最近被命名为GST 12。此外，基因组Southern印迹数据表明人类微粒体GST由单拷贝或极低拷贝基因编码。因此，人类GST基因超家族至少位于四条不同的染色体上：1号染色体（GST 1）、6号染色体（GST 2）、11号染色体（GST 3）和12号染色体（GST 12）。
