利用内含子特异性聚合酶链反应对人类谷胱甘肽S-转移酶μ基因（GSTM1）进行染色体定位和连锁分析。
Chromosomal assignment and linkage analysis of the human glutathione S-transferase mu gene (GSTM1) using intron specific polymerase chain reaction.
作者信息
Zhong S, Wolf C R, Spurr N K
机构信息
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK.
出版信息
Hum Genet. 1992 Dec;90(4):435-9. doi: 10.1007/BF00220473.
PMID:1483702
Abstract
Oligonucleotide primers specific for intron 5 sequences were used to amplify a unique 718 bp fragment in the human GST mu gene. Using DNA from a panel of somatic cell hybrids it was possible to confirm the assignment of the GST1 locus to chromosome 1p and to refine localisation to 1p13 using Southern blot analysis of DNA from three-generation CEPH families and a GST mu specific DNA probe.
摘要
针对人谷胱甘肽S-转移酶μ基因第5内含子序列的寡核苷酸引物，用于扩增该基因中一段独特的718 bp片段。利用一组体细胞杂种的DNA，通过对三代CEPH家系的DNA进行Southern印迹分析，并使用谷胱甘肽S-转移酶μ特异性DNA探针，得以确认谷胱甘肽S-转移酶1（GST1）基因座定位于染色体1p，并将其定位精确至1p13。
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