无机磷酸盐对纳那霉素生物合成的调控位点。

Site of regulation of nanaomycin biosynthesis by inorganic phosphate.

作者信息

Masuma R, Zhen D Z, Tanaka Y, Omura S

机构信息

Kitasato Institute, Kitasato University, Tokyo, Japan.

出版信息

J Antibiot (Tokyo). 1990 Jan;43(1):83-7. doi: 10.7164/antibiotics.43.83.

DOI:10.7164/antibiotics.43.83

PMID:2307632

Abstract

The site of regulation of nanaomycin biosynthesis by inorganic phosphate was studied with washed cells previously grown in a chemically defined medium containing a high- or low-phosphate concentration. The former mycelia produced only about one-tenth the amount of nanaomycin A from acetate as did the latter mycelia. On the other hand, the bioconversions of nanaomycin D to A and nanaomycin A to E were only slightly affected. It is suggested that the site of regulation of nanaomycin biosynthesis by inorganic phosphate lies within steps between acetate and nanaomycin D.

摘要

利用先前在含有高磷或低磷浓度的化学成分确定的培养基中生长的洗涤细胞，研究了无机磷酸盐对纳那霉素生物合成的调控位点。前一种菌丝体从乙酸盐产生的纳那霉素A的量仅为后一种菌丝体的十分之一左右。另一方面，纳那霉素D向A以及纳那霉素A向E的生物转化仅受到轻微影响。表明无机磷酸盐对纳那霉素生物合成的调控位点位于乙酸盐和纳那霉素D之间的步骤中。