Department of Chemistry, 336 BB, University of Iowa, Iowa City, Iowa 52242, USA.
ACS Chem Neurosci. 2012 Oct 17;3(10):761-6. doi: 10.1021/cn300061z. Epub 2012 Jul 4.
Ionic zinc is found at a high concentration in some glutamatergic vesicles of the mammalian brain. Ionic zinc is also found chelated to macromolecules in the extracellular space, constituting what has been called the "zinc veneer". In this communication we show that the zinc ionophore, pyrithione, can be used to demonstrate the presence of the veneer. Application of pyrithione without added ionic zinc to rodent hippocampal slices mobilizes extracellular zinc, which can be detected intracellularly by the zinc probe FluoZin-3. In addition, we show that ZnT3 null mice, which lack the transporter responsible for stocking synaptic vesicles, nevertheless do have a zinc veneer, albeit diminished compared to wild type animals. The presence of the zinc veneer in ZnT3 null mice may account for the absence of any marked deficit in these animals.
离子锌在哺乳动物大脑的一些谷氨酸能小泡中高度集中。离子锌也与细胞外空间中的大分子螯合，构成了所谓的“锌层”。在本通讯中，我们表明锌离子载体吡硫翁可以用来证明锌层的存在。将没有添加离子锌的吡硫翁应用于啮齿动物海马切片中，可以动员细胞外锌，锌探针 FluoZin-3 可以在细胞内检测到。此外，我们还表明，ZnT3 缺失小鼠缺乏负责储存突触小泡的转运体，但它们确实有锌层，尽管与野生型动物相比有所减少。ZnT3 缺失小鼠中锌层的存在可能解释了这些动物没有明显缺陷的原因。
