Mesa V A, De Groote J, Fevery J
Department of Medical Research, University of Leuven, Belgium.
J Hepatol. 1990 Jan;10(1):35-40. doi: 10.1016/0168-8278(90)90070-8.
The effect of organic anions on bilirubin metabolism and excretion was investigated in rats. Biliary excretion of bromosulfophthalein, bromcresol green and indocyanine green led to a significant decrease in excretion of bilirubin pigments and to an increase of their concentration in serum. This was concomitant with a marked increase in the ratio of bilirubin diconjugates to monoconjugates in bile and serum. These changes were unrelated to either bile flow, biliary lipid output, or hepatic activity of bilirubin UDP-glucuronyl transferase. Following bolus injection of [14C]bilirubin, peak excretory rate of radioactivity was markedly delayed in rats infused with bromosulfophthalein, as compared to controls. It is concluded that administration of the organic anions increased the ratio of bilirubin diconjugates to monoconjugates in bile and serum, by slowing down the intrahepatic transit of bilirubin pigments. This, in turn, allowed more efficient enzyme-catalyzed formation of bilirubin diconjugates from the intermediate bilirubin monoconjugates.
研究了有机阴离子对大鼠胆红素代谢和排泄的影响。溴磺酞钠、溴甲酚绿和吲哚菁绿的胆汁排泄导致胆红素色素排泄显著减少，血清中其浓度升高。这伴随着胆汁和血清中胆红素双结合物与单结合物比例的显著增加。这些变化与胆汁流量、胆汁脂质输出或胆红素UDP-葡萄糖醛酸转移酶的肝脏活性均无关。与对照组相比，在推注[14C]胆红素后，输注溴磺酞钠的大鼠放射性物质的峰值排泄率明显延迟。得出的结论是，有机阴离子的给药通过减缓胆红素色素在肝内的转运，增加了胆汁和血清中胆红素双结合物与单结合物的比例。这反过来又使中间胆红素单结合物能更有效地通过酶催化形成胆红素双结合物。
