Department of Biochemistry, Medical University of Gdansk, Debinki 1, 80-211 Gdansk, Poland.
J Steroid Biochem Mol Biol. 2013 Mar;134:37-44. doi: 10.1016/j.jsbmb.2012.10.006. Epub 2012 Oct 16.
Contradictory results have been published regarding the influence of progesterone on lipids metabolism in adipose tissue. The aim of the present work was to elucidate whether progesterone administration in the setting of an experimental model influences lipogenic enzyme genes expression, body and adipose tissue mass. The results presented here indicate that the elevated blood progesterone concentration was associated with significant increase in lipogenic enzyme genes expression in inguinal adipose tissue of females. The rise in the expression of lipogenic enzyme genes was associated with an increase in sterol regulatory element binding transcription factor 1 (Srebf1) and S14 genes expression. Mifepristone, a specific antagonist of progesterone receptor, abolished progesterone's effect on body mass, inguinal fat mass, and lipogenic enzyme genes expression in inguinal adipose tissue. No significant changes were found in the expression of lipogenic enzyme genes, Srebf1 and S14 genes in perirenal white adipose tissue of females. The elevated blood progesterone concentration was associated with the increase in body and inguinal white adipose tissue mass of females. In males, elevated blood progesterone concentration had no effect on the lipogenic enzyme genes expression and on body and fat mass. In conclusion, we demonstrate that a chronic increase in serum progesterone concentration in females was associated with up-regulation of lipogenic enzyme genes expression in inguinal adipose tissue. Up-regulation of Srebf1 and S14 genes expression following progesterone administration suggests that products of these genes might be involved in the regulation of lipogenic enzyme genes expression by progesterone. The stimulatory effect of progesterone on lipogenic enzyme genes expression in inguinal adipose tissue seems to be specific as it was reversed by specific antagonist of progesterone receptor.
关于孕激素对脂肪组织脂代谢的影响，已有相互矛盾的研究结果发表。本研究旨在阐明在实验模型中给予孕激素是否会影响脂肪生成酶基因的表达、体重和脂肪组织量。本研究结果表明，血液中孕激素浓度的升高与雌性腹股沟脂肪组织中脂肪生成酶基因表达的显著增加有关。脂肪生成酶基因表达的增加与固醇调节元件结合转录因子 1（Srebf1）和 S14 基因表达的增加有关。米非司酮是孕激素受体的特异性拮抗剂，可消除孕激素对体重、腹股沟脂肪量和腹股沟脂肪组织中脂肪生成酶基因表达的影响。在雌性肾周白色脂肪组织中，未发现脂肪生成酶基因、Srebf1 和 S14 基因表达有显著变化。血液中孕激素浓度的升高与雌性体重和腹股沟白色脂肪组织量的增加有关。在雄性中，血液中孕激素浓度的升高对脂肪生成酶基因表达以及体重和脂肪量没有影响。总之，我们证明了女性血清孕激素浓度的慢性升高与腹股沟脂肪组织中脂肪生成酶基因表达的上调有关。孕激素给药后 Srebf1 和 S14 基因表达的上调表明这些基因的产物可能参与孕激素对脂肪生成酶基因表达的调节。孕激素对腹股沟脂肪组织中脂肪生成酶基因表达的刺激作用似乎是特异性的，因为它可以被孕激素受体的特异性拮抗剂逆转。
