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甲状腺激素反应性 SPOT14 基因的表达受雌激素在鸡（Gallus gallus）中的调控。

Expression of Thyroid Hormone Responsive SPOT 14 Gene Is Regulated by Estrogen in Chicken (Gallus gallus).

机构信息

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.

Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Henan Agricultural University, Zhengzhou, 450002, China.

出版信息

Sci Rep. 2017 Aug 31;7(1):10243. doi: 10.1038/s41598-017-08452-6.

DOI:10.1038/s41598-017-08452-6
PMID:28860448
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5579026/
Abstract

Thyroid hormone responsive spot 14 (THRSP) is a small nuclear protein that responds rapidly to thyroid hormone. It has been shown that THRSP is abundant in lipogenic tissues such as liver, fat and the mammary gland in mammals. The THRSP gene acts as a key lipogenic activator and can be activated by thyroid hormone triiodothyronine (T3), glucose, carbohydrate and insulin. Here we report that chicken THRSP is also abundant in lipogenic tissues including the liver and the abdominal fat, and its expression levels increased with sex maturation and reached the highest level at the peak of egg production. Structure analysis of the THRSP gene indicates that there is a conscious estrogen response element (ERE) located in the -2390 - -2402 range of the gene promoter region. Further studies by ChIP-qPCR proved that the ERα interacts with the putative ERE site. In addition, THRSP was significantly upregulated (P < 0.05) when chickens or chicken primary hepatocytes were treated with 17β-estradiol in both the in vivo and in vitro conditions. We therefore conclude that THRSP is directly regulated by estrogen and is involved in the estrogen regulation network in chicken.

摘要

甲状腺激素反应元件结合蛋白 14（THRSP）是一种对甲状腺激素快速反应的小核蛋白。已证实，THRSP 在哺乳动物的肝、脂肪和乳腺等产脂组织中含量丰富。THRSP 基因作为关键的产脂激活剂，可被甲状腺激素三碘甲状腺原氨酸（T3）、葡萄糖、碳水化合物和胰岛素激活。本研究报告表明，鸡 THRSP 在产脂组织中也大量存在，包括肝脏和腹部脂肪，其表达水平随着性成熟而增加，并在产蛋高峰期达到最高水平。THRSP 基因结构分析表明，在基因启动子区域的-2390 到-2402 范围内存在一个有意识的雌激素反应元件（ERE）。进一步的 ChIP-qPCR 研究证明 ERα 与推定的 ERE 位点相互作用。此外，在体内和体外条件下，用 17β-雌二醇处理鸡或鸡原代肝细胞时，THRSP 显著上调（P<0.05）。因此，我们得出结论，THRSP 受雌激素直接调控，并参与鸡的雌激素调控网络。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/de9ab67ba2cb/41598_2017_8452_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/6e418ada5699/41598_2017_8452_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/b0cbdc1d6a7d/41598_2017_8452_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/b375fef8488f/41598_2017_8452_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/8706e68d8337/41598_2017_8452_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/c54791d34f55/41598_2017_8452_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/36e58f1f6c60/41598_2017_8452_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/13468392e90e/41598_2017_8452_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/de9ab67ba2cb/41598_2017_8452_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/6e418ada5699/41598_2017_8452_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/b0cbdc1d6a7d/41598_2017_8452_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/b375fef8488f/41598_2017_8452_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/8706e68d8337/41598_2017_8452_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/c54791d34f55/41598_2017_8452_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/36e58f1f6c60/41598_2017_8452_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/13468392e90e/41598_2017_8452_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/5579026/de9ab67ba2cb/41598_2017_8452_Fig8_HTML.jpg

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