Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Biotechnol Lett. 2013 May;35(5):799-810. doi: 10.1007/s10529-012-1130-1. Epub 2013 Jan 13.
Malaysian rice, Pongsu Seribu 2, has wide-spectrum resistance against blast disease. Chromosomal locations conferring quantitative resistance were detected by linkage mapping with SSRs and quantitative trait locus (QTL) analysis. For the mapping population, 188 F3 families were derived from a cross between the susceptible cultivar, Mahsuri, and a resistant variety, Pongsu Seribu 2. Partial resistance to leaf blast in the mapping population was assessed. A linkage map covering ten chromosomes and consisting of 63 SSR markers was constructed. 13 QTLs, including 6 putative and 7 putative QTLs, were detected on chromosomes 1, 2, 3, 5, 6, 10, 11 and 12. The resulting phenotypic variation due to a single QTL ranged from 2 to 13 %. These QTLs accounted for approx. 80 % of the total phenotypic variation within the F3 population. Therefore, partial resistance to blast in Pongsu Seribu 2 is due to combined effects of multiple loci with major and minor effects.
马来西亚水稻品种蓬速 2 号对稻瘟病具有广谱抗性。利用 SSR 标记和数量性状位点（QTL）分析，对赋予其定量抗性的染色体位置进行连锁作图检测。在作图群体中，利用感病品种 Mahsuri 和抗源蓬速 2 号杂交衍生出 188 个 F3 家系。对该作图群体的叶片稻瘟病的部分抗性进行评估。构建了一张包含十个染色体、由 63 个 SSR 标记组成的连锁图谱。在染色体 1、2、3、5、6、10、11 和 12 上检测到 13 个 QTL，包括 6 个推定 QTL 和 7 个推定 QTL。单个 QTL 引起的表型变异幅度为 2-13%。这些 QTL 解释了 F3 群体中约 80%的总表型变异。因此，蓬速 2 号对稻瘟病的部分抗性是由多个主效和微效位点的综合作用引起的。
