Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK.
Org Biomol Chem. 2012 Dec 7;10(45):9021-9. doi: 10.1039/c2ob25751c. Epub 2012 Oct 19.
The uses of 3-formylbenzoic acid and 4-formylbenzoic acid as molecular probes along with previous and new transketolase mutants revealed the factors governing the rate of reaction between transketolase and aromatic aldehydes. The novel α,α-dihydroxyketones were produced at 15 to 30-fold higher yields and up to 250-fold higher specific activities with D469T TK when compared to those obtained for benzaldehyde.
3-甲酰苯甲酸和 4-甲酰苯甲酸作为分子探针的用途，以及以前和新的转酮醇酶突变体的用途，揭示了控制转酮醇酶与芳香醛之间反应速率的因素。与苯甲醛相比，新型 α,α-二羟基酮的产率提高了 15 至 30 倍，比活度提高了 250 倍。当使用 D469T TK 时。
