用于亚马逊地区（Astrocaryum aculeatum）图库玛的微卫星位点以及在其他棕榈科中的扩增。
Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae.
机构信息
Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, CP 83, CEP 13400-970, Piracicaba, São Paulo, Brazil.
出版信息
Am J Bot. 2012 Dec;99(12):e508-10. doi: 10.3732/ajb.1100607. Epub 2012 Dec 4.
PREMISE OF THE STUDY
Microsatellite loci were developed for tucumã of Amazonas (Astrocaryum aculeatum), and cross-species amplification was performed in six other Arecaceae, to investigate genetic diversity and population structure and to provide support for natural populations management. •
METHODS AND RESULTS
Fourteen microsatellite loci were isolated from a microsatellite-enriched genomic library and used to characterize two wild populations of tucumã of Amazonas (Manaus and Manicoré cities). The investigated loci displayed high polymorphism for both A. aculeatum populations, with a mean observed heterozygosity of 0.498. Amplification rates ranging from 50% to 93% were found for four Astrocaryum species and two additional species of Arecaceae. •
CONCLUSIONS
The information derived from the microsatellite markers developed here provides significant gains in conserved allelic richness and supports the implementation of several molecular breeding strategies for the Amazonian tucumã.
研究前提
为亚马孙树榈（Astrocaryum aculeatum）开发了微卫星位点，并在其他六种棕榈科植物中进行了跨物种扩增，以调查遗传多样性和种群结构，并为自然种群管理提供支持。
方法和结果
从微卫星富集基因组文库中分离出 14 个微卫星位点，用于表征亚马孙地区的两个野生树榈种群（玛瑙斯和马瑙雷市）。研究发现，这两个树榈种群的 14 个微卫星位点具有高度多态性，平均观测杂合度为 0.498。四种 Astrocaryum 物种和两种其他棕榈科植物的扩增率范围为 50%至 93%。
结论
这里开发的微卫星标记所提供的信息在保守等位基因丰富度方面有显著提高，并支持实施几种针对亚马逊地区树榈的分子育种策略。
Zotero 插件