Chiu A T, Ryan J W, Bryan F T, Niemeyer R E, Schultz D R
Biochem J. 1979 Oct 15;184(1):7-12. doi: 10.1042/bj1840007.
The present study attempts to determine if the isolated rat liver is capable of synthesizing renin substrate from 14C-labelled amino acids added in the perfusate. The renin substrate is characterized via reaction with renin, forming a substance that is subsequently identified as proangiotensin. Extensive evaluation of the reaction product is carried out by using molecular-sieve chromatography, countercurrent distribution, reactivity with converting enzyme, radioimmunological technique and bioassay. The results demonstrate that isolated rat liver perfused with artificial salt solution is capable of synthesizing a protein that reacts with renin to form a radioactive substance indistinguishable from proangiotensin.
本研究试图确定离体大鼠肝脏是否能够从灌注液中添加的14C标记氨基酸合成肾素底物。通过与肾素反应来表征肾素底物，形成一种随后被鉴定为血管紧张素原的物质。利用分子筛色谱、逆流分配、与转化酶的反应性、放射免疫技术和生物测定法对反应产物进行了广泛评估。结果表明，用人工盐溶液灌注的离体大鼠肝脏能够合成一种与肾素反应形成与血管紧张素原无法区分的放射性物质的蛋白质。
