Institute of Human Nutrition and Developmental Origins of Health and Disease Division, School of Medicine, University of Southampton, Southampton SO16 6YD, United Kingdom.
Clin Nutr. 2010 Aug;29(4):519-24. doi: 10.1016/j.clnu.2009.11.004. Epub 2009 Nov 28.
BACKGROUND & AIMS: An in vitro study showed that a lipid emulsion containing fish oil (FO) slows the growth of colon cancer cells and enhances their sensitivity to 5-fluorouracil (FU). The aim was to confirm this finding and to compare such an emulsion with an alternative to lowered n-6 fatty acid exposure.
We determined the number of viable cells, apoptosis and cell cycle distribution of HT-29 cells after exposure to one of three lipid emulsions. Cell cycle distribution was also assessed after treatment with lipid emulsions and FU.
The lipid emulsion containing FO induced a significant growth inhibitory effect without changing the percentage of apoptotic cells. Exposure to the other lipid emulsions had no effect on growth and decreased apoptosis. Each lipid emulsion potentiated the S phase-halting effect of 1 and 10microM FU. This effect also occurred at 0.1microM FU when the cells were exposed to the FO containing lipid emulsion.
A lipid emulsion containing FO has a growth inhibitory effect on a human colon adenocarcinoma cell line, an effect not due to the induction of apoptosis, and potentiated the S phase-halting effect of FU. Thus, an FO lipid emulsion may be of benefit in colorectal cancer.
一项体外研究表明，含有鱼油（FO）的脂肪乳剂可减缓结肠癌细胞的生长速度，并增强其对氟尿嘧啶（FU）的敏感性。本研究旨在证实这一发现，并比较这种乳剂与降低 n-6 脂肪酸暴露的替代物。
我们测定了 HT-29 细胞在接触三种脂肪乳剂之一后活细胞数量、细胞凋亡和细胞周期分布的变化。还评估了用脂肪乳剂和 FU 处理后细胞周期分布的变化。
含 FO 的脂肪乳剂诱导了明显的生长抑制作用，而不改变细胞凋亡的百分比。其他两种脂肪乳剂对生长没有影响，但会减少细胞凋亡。每种脂肪乳剂都增强了 1 和 10μM FU 的 S 期阻断作用。当细胞接触含 FO 的脂肪乳剂时，这种作用也发生在 0.1μM FU 时。
含 FO 的脂肪乳剂对人结肠腺癌细胞系具有生长抑制作用，这种作用不是通过诱导细胞凋亡引起的，并且增强了 FU 的 S 期阻断作用。因此，FO 脂肪乳剂可能对结直肠癌有益。
