Department of Laboratory Medicine, University of California, San Francisco, CA, United States.
Clin Chim Acta. 2013 May;420:4-10. doi: 10.1016/j.cca.2012.10.026. Epub 2012 Oct 22.
For certain clinical chemistry and toxicology analytes, liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) offers significant advantages over traditional testing by immunoassay.
Published reports comparing the performance of immunoassays against LC-MS/MS were reviewed. The tested analytes include testosterone, estradiol, thyroid hormones, vitamin D, immunosuppressants, steroids for newborn screening programs, and clinical and forensic toxicology.
While immunoassays are widely used in the clinical laboratory, the analytical sensitivity and specificity are inferior for many of the analytes tested in routine clinical laboratories. Moreover, LC-MS/MS can be multiplexed for high testing throughput and multiple analyte detection. The disadvantages of LC-MS/MS include the absence of Food and Drug Administration (FDA) and Conformité Européenne (CE) Mark approved tests, the high cost of analytical instrumentation, and the technical expertise needed to operate and maintain analyzers.
The implementation of LC-MS/MS will increase in the next few years as this technology continues to improve and the advantages become more well known.
对于某些临床化学和毒理学分析物，液相色谱（LC）与串联质谱（MS/MS）联用相对于传统的免疫测定具有显著优势。
综述了比较免疫测定与 LC-MS/MS 性能的已发表报告。所测试的分析物包括睾酮、雌二醇、甲状腺激素、维生素 D、免疫抑制剂、新生儿筛查计划中的类固醇以及临床和法医毒理学。
尽管免疫测定在临床实验室中得到广泛应用，但对于常规临床实验室中测试的许多分析物，其分析灵敏度和特异性较差。此外，LC-MS/MS 可进行多重检测，以实现高通量测试和多种分析物检测。LC-MS/MS 的缺点包括缺乏食品和药物管理局（FDA）和欧洲合格评定（CE）标记批准的测试、分析仪器成本高，以及操作和维护分析仪所需的技术专业知识。
随着该技术的不断改进和优势的广为人知，未来几年内 LC-MS/MS 的应用将增加。
