Laboratorio de Comunidades Marinas y Ecotoxicología, Departamento Biología de Organismos, Universidad Simón Bolívar, Apdo. 8900, Caracas, 1080-A, Venezuela.
Bull Environ Contam Toxicol. 2013 Jan;90(1):1-8. doi: 10.1007/s00128-012-0862-x. Epub 2012 Oct 20.
The objective of this work was to evaluate the potential use of the bivalve Tivela mactroides, as a Caribbean sentinel species. Organisms were collected from a relatively clean sandy beach, and were exposed to a gradient of petroleum hydrocarbon-polluted sediments for periods of 3, 4 and 6 days, after which their activity levels of biotransformation enzymes were measured. NADPH-cytochrome c reductase, glutathione transferase and cytochrome b5 activities increased according to total hydrocarbon concentration, whereas NADH-cytochrome ferricyanide reductase activity decreased. Catalase activity was constant while superoxide dismutase activity was inhibited. The results indicated that T. mactroides may serve as a sentinel species. However, further studies are recommended on the influence of biotic and abiotic variables on basal activities of enzymes, and their responses to other pollutants.
本研究旨在评估双壳类软体动物 Tivela mactroides 作为加勒比海指示物种的潜在用途。从相对清洁的沙滩采集生物样本，并将其暴露于石油烃污染沉积物的梯度中 3、4 和 6 天，之后测量其生物转化酶的活性水平。NADPH-细胞色素 c 还原酶、谷胱甘肽转移酶和细胞色素 b5 的活性随总烃浓度的增加而增加，而 NADH-细胞色素铁氰化物还原酶的活性则降低。过氧化氢酶的活性保持不变，而超氧化物歧化酶的活性受到抑制。结果表明，T. mactroides 可作为指示物种。然而，建议进一步研究生物和非生物变量对酶基础活性的影响及其对其他污染物的反应。
