Department of Pharmaceutical Sciences and Drug Research, Punjabi University, India.
J Renin Angiotensin Aldosterone Syst. 2013 Jun;14(2):116-23. doi: 10.1177/1470320312460899. Epub 2012 Oct 19.
The present study was designed to investigate the potential of aliskiren, a direct renin inhibitor, in chronic constriction injury (CCI)-induced neuropathic pain in rats. Neuropathic pain was induced by placing four loose ligatures around the sciatic nerve. Acetone drop, von Frey hair, pin-prick and hot plate tests were performed to assess cold allodynia, mechanical allodynia, mechanical and heat hyperalgesia, respectively. The levels of Tumor necrosis factor-alpha (TNF-α) were measured in the sciatic nerve as an inflammatory marker. CCI was associated with the development of cold allodynia, mechanical allodynia, mechanical and heat hyperalgesia along with a rise in the levels of Tumor necrosis factor-alpha (TNF-α). Administration of aliskiren (25 or 50 mg/kg intraperitoneal (i.p.)) for 14 days in CCI-subjected rats significantly attenuated CCI-induced pain-related behavior and rise in TNF-α level. It may be concluded that aliskiren-mediated anti-inflammatory actions may be responsible for its beneficial effects in neuropathic pain in rats.
本研究旨在探讨直接肾素抑制剂阿利克仑在大鼠慢性缩窄性损伤（CCI）诱导的神经性疼痛中的潜力。通过在坐骨神经周围放置四根松动的结扎线来诱导神经性疼痛。使用丙酮滴注、von Frey 毛发、针刺和热板试验分别评估冷感觉过敏、机械性感觉过敏、机械性和热痛觉过敏。测量坐骨神经中肿瘤坏死因子-α（TNF-α）的水平作为炎症标志物。CCI 与冷感觉过敏、机械性感觉过敏、机械性和热痛觉过敏的发展以及肿瘤坏死因子-α（TNF-α）水平的升高有关。在 CCI 处理的大鼠中腹腔内给予阿利克仑（25 或 50mg/kg）14 天可显著减轻 CCI 诱导的疼痛相关行为和 TNF-α水平的升高。可以得出结论，阿利克仑介导的抗炎作用可能是其在大鼠神经性疼痛中有益作用的原因。
