Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran.
Medical Toxicology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Metab Brain Dis. 2022 Dec;37(8):2839-2852. doi: 10.1007/s11011-022-01094-2. Epub 2022 Oct 12.
Neuropathic pain, a chronic pain condition, puts a considerable burden on its patients. However, different pathophysiological characteristics of neuropathic pain make the current treatment medications insufficient in controlling pain. Identifying treatment effects with Capparis Spinosa hydro-alcoholic extract in an animal model of neuropathic pain. Liquid chromatography-mass spectrometry (LC-MS) was used to identify the components of C. Spinosa hydro-alcoholic extract. To establish a neuropathic pain model, adult male Wistar rats underwent chronic constriction injury (CCI) surgery in their left sciatic nerve. Male wistar rats were divided into four groups: CCI, Sham, CCI with C. Spinosa (100 mg/kg), and CCI with C. Spinosa (200 mg/kg). Rats were treated with a hydro-alcoholic extract from aerial parts of the C. Spinosa (orally, daily) starting from CCI induction until 14 days after. Behavioral tests (mechanical allodynia, cold allodynia, and thermal hyperalgesia) and biochemical tests (IL-1β, TNF-α, MDA, and total thiol) were taken from animals. The LC-MS analysis identified 22 compounds in C. Spinosa extract with the predominance of flavonoids. CCI produced a significant (P < 0.001) increase allodynia (mechanical and cold) and thermal hyperalgesia in comparison with sham group. Oral administration of C. Spinosa significantly (P < 0.05) ameliorated CCI-induced nociceptive pain compared with CCI group. Spinal cord specimens of CCI rats had significant (P < 0.05) elevated inflammation status (↑IL-1β, ↑TNF-α), and significant (P < 0.05) decreased antioxidative status (↑MDA, ↓total thiol) in comparison with the sham group. These changes were reversed following C. Spinosa treatment. C. Spinosa alleviates neuropathic pain by exhibiting antioxidative and anti-inflammatory effects. The responsible components for these effects are possibly the flavonoid compounds in C. Spinosa extract.
神经病理性疼痛是一种慢性疼痛病症，给患者带来了相当大的负担。然而，神经病理性疼痛的不同病理生理特征使得目前的治疗药物在控制疼痛方面效果不足。本研究旨在评估在神经病理性疼痛动物模型中，刺山柑水醇提取物的治疗效果。采用液相色谱-质谱联用（LC-MS）技术鉴定刺山柑水醇提取物的成分。通过对成年雄性 Wistar 大鼠左侧坐骨神经进行慢性缩窄性损伤（CCI）手术，建立神经病理性疼痛模型。雄性 Wistar 大鼠分为 4 组：CCI 组、假手术（Sham）组、CCI 加刺山柑 100mg/kg 组和 CCI 加刺山柑 200mg/kg 组。从 CCI 诱导开始至 14 天后，每天用刺山柑地上部分的水醇提取物（灌胃）处理大鼠。对动物进行行为学测试（机械性痛觉过敏、冷感觉过敏和热痛觉过敏）和生化测试（IL-1β、TNF-α、MDA 和总巯基）。LC-MS 分析鉴定出刺山柑提取物中的 22 种化合物，其中以类黄酮为主。与 Sham 组相比，CCI 组显著增加了机械性和冷感觉过敏（P<0.001）和热痛觉过敏。与 CCI 组相比，刺山柑给药显著改善了 CCI 诱导的痛觉过敏（P<0.05）。与 Sham 组相比，CCI 大鼠脊髓标本中炎症状态明显升高（↑IL-1β、↑TNF-α），抗氧化状态明显降低（↑MDA、↓总巯基）（P<0.05）。这些变化在刺山柑治疗后得到逆转。刺山柑通过抗氧化和抗炎作用缓解神经病理性疼痛。这些作用的可能与刺山柑提取物中的黄酮类化合物有关。
