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基质辅助激光解吸电离/飞行时间质谱法用于临床诊断。

Matrix-assisted laser desorption ionization/time-of-flight mass spectrometry for clinical diagnosis.

机构信息

Department of Cosmetic Applications and Management, Yuh-Ing Junior College of Health Care & Management, Kaohsiung, Taiwan.

出版信息

Clin Chim Acta. 2013 Jan 16;415:266-75. doi: 10.1016/j.cca.2012.10.032. Epub 2012 Oct 23.

DOI:10.1016/j.cca.2012.10.032
PMID:23089072
Abstract

BACKGROUND

Matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) mass spectrometry is known as an extremely sensitive analytical tool for characterizing different types of biological compounds including proteins, peptides and lipids. Since MALDI-TOF analysis requires very simple sample pretreatment, the technique can be used for rapidly detecting biochemical compounds serving as disease biomarkers.

RESULTS

This mini-review focuses on the applications of MALDI-TOF in the detection of potential disease biomarkers in various biological samples.

CONCLUSIONS

The potential disease biomarkers are mostly abundant proteins, peptides, or lipids including: albumin; hemoglobin; α-defensins; trimethylamine; phospholipids; and glycated α- and β-globin, which are indicators of albuminuria; fecal occult blood and ischemic stroke; dry eye disease and/or aging; trimethylaniuria; breast cancer; and diabetes, respectively.

摘要

背景

基质辅助激光解吸电离/飞行时间（MALDI-TOF）质谱分析被认为是一种非常敏感的分析工具，可用于鉴定包括蛋白质、肽和脂质在内的不同类型的生物化合物。由于 MALDI-TOF 分析只需要非常简单的样品预处理，因此该技术可用于快速检测作为疾病生物标志物的生化化合物。

结果

本综述聚焦于 MALDI-TOF 在各种生物样本中检测潜在疾病生物标志物的应用。

结论

潜在的疾病生物标志物大多为丰富的蛋白质、肽或脂质，包括：白蛋白；血红蛋白；α-防御素；三甲胺；磷脂；糖化的α-和β-球蛋白，分别是白蛋白尿、粪便潜血和缺血性脑卒中、干眼症和/或衰老、三甲基尿酸、乳腺癌和糖尿病的标志物。

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