Department of Applied Science, National Taitung University, 684 Section 1, Chunghua Road, Taitung 95002, Taiwan.
Int J Mol Sci. 2014 Apr 28;15(5):7266-80. doi: 10.3390/ijms15057266.
Identifying trace amounts of bacteria rapidly, accurately, selectively, and with high sensitivity is important to ensuring the safety of food and diagnosing infectious bacterial diseases. Microbial diseases constitute the major cause of death in many developing and developed countries of the world. The early detection of pathogenic bacteria is crucial in preventing, treating, and containing the spread of infections, and there is an urgent requirement for sensitive, specific, and accurate diagnostic tests. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an extremely selective and sensitive analytical tool that can be used to characterize different species of pathogenic bacteria. Various functionalized or unmodified nanomaterials can be used as affinity probes to capture and concentrate microorganisms. Recent developments in bacterial detection using nanomaterials-assisted MALDI-MS approaches are highlighted in this article. A comprehensive table listing MALDI-MS approaches for identifying pathogenic bacteria, categorized by the nanomaterials used, is provided.
快速、准确、选择性地检测痕量细菌，并具有高灵敏度，对于确保食品安全和诊断传染性细菌疾病非常重要。微生物疾病是世界上许多发展中国家和发达国家死亡的主要原因。早期检测致病菌对于预防、治疗和控制感染的传播至关重要，因此迫切需要敏感、特异和准确的诊断检测方法。基质辅助激光解吸/电离质谱（MALDI-MS）是一种非常有选择性和灵敏的分析工具，可用于鉴定不同种类的致病菌。各种功能化或未修饰的纳米材料可用作亲和探针来捕获和浓缩微生物。本文重点介绍了利用纳米材料辅助 MALDI-MS 方法进行细菌检测的最新进展。提供了一个包含使用的纳米材料分类的 MALDI-MS 方法识别致病菌的综合表格。
