Faculty of Pharmacy, Universidade Federal de Minas Gerais, Brazil.
Clin Chim Acta. 2013 Jan 16;415:226-9. doi: 10.1016/j.cca.2012.10.006. Epub 2012 Oct 23.
There may be a relationship between endothelial dysfunction, coagulation activation and nitric oxide (NO) synthesis in women with mild and severe preeclampsia (PE).
Plasma thrombomodulin (TM), D-Dimer (D-Di), cyclic guanosine monophosphate (cGMP) and placental nitric oxide synthase activity (NOS) were investigated in 21 normotensive pregnant women (G1), 22 pregnant women with mild PE (G2) and 20 pregnant women with severe PE (G3).
TM and D-Di were significantly increased in G3 compared to G1 (P=0.001 and P=0.006, respectively) and G2 (P=0.001, in both cases). However, there was no significant difference when G1 was compared to G2. For total NOS, calcium independent NOS, calcium dependent NOS no significant difference was observed among the groups studied.
TM and D-Di levels are raised in women with severe PE compared to normotensive pregnant women and women with mild PE. While increased TM levels may reflect endothelial dysfunction, raised D-Di levels indicate a hypercoagulable state. NO assessed by 2 indirect methods did not show any significant difference among the groups studied. Due to current limitations with in vitro NO measurements and interferences associated with NO bioavailability, particularly in PE, such findings should not be over-interpreted.
轻度和重度子痫前期（PE）妇女的内皮功能障碍、凝血激活和一氧化氮（NO）合成之间可能存在关系。
研究了 21 名正常血压孕妇（G1）、22 名轻度 PE 孕妇（G2）和 20 名重度 PE 孕妇（G3）的血浆血栓调节蛋白（TM）、D-二聚体（D-Di）、环鸟苷单磷酸（cGMP）和胎盘一氧化氮合酶活性（NOS）。
与 G1（P=0.001 和 P=0.006）和 G2（P=0.001，均如此）相比，G3 中的 TM 和 D-Di 显著增加。然而，G1 与 G2 之间没有显著差异。对于总 NOS、钙非依赖性 NOS、钙依赖性 NOS，各组之间没有观察到显著差异。
与正常血压孕妇和轻度 PE 孕妇相比，重度 PE 妇女的 TM 和 D-Di 水平升高。虽然 TM 水平升高可能反映内皮功能障碍，但 D-Di 水平升高表明存在高凝状态。通过 2 种间接方法评估的 NO 并没有显示出各组之间有任何显著差异。由于目前体外 NO 测量存在局限性，以及与 NO 生物利用度相关的干扰，特别是在 PE 中，这些发现不应被过度解释。
