Technology Transfer Unit, Specialist Scientific Services Department, Animal Health and Veterinary Laboratories Agency - Weybridge, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK.
J Immunol Methods. 2013 Jan 31;387(1-2):140-6. doi: 10.1016/j.jim.2012.10.007. Epub 2012 Oct 23.
An assay for detection of antibodies to bovine herpesvirus-1 (BoHV-1) was developed by combining a commercial low cost, disposable biosensor system (Vantix™) and reagents from an established Enzyme-Linked Immunosorbent Assay (ELISA). The biosensor assay produced equivalent results to ELISA within 15 min when testing 194 bovine serum and 50 bulk milk samples submitted for routine testing. The biosensor assay can provide quantitative analysis demonstrated by measuring the level of antibody in milk samples. The results of this study suggest that Vantix™ is a promising platform for routine immunological testing. The technology may be particularly useful for low to medium throughput tests where rapid results are required. The biosensors could also form the basis of a future point-of-care test platform.
开发了一种用于检测牛疱疹病毒 1 型（BoHV-1）抗体的检测方法，该方法将商业上低成本、一次性的生物传感器系统（Vantix™）与一种已建立的酶联免疫吸附测定（ELISA）试剂相结合。当测试 194 份牛血清和 50 份批量牛奶样本时，生物传感器检测在 15 分钟内产生了与 ELISA 相当的结果。生物传感器检测可以通过测量牛奶样本中的抗体水平提供定量分析。这项研究的结果表明，Vantix™是一种很有前途的常规免疫检测平台。该技术可能特别适用于需要快速结果的低到中通量测试。生物传感器还可以作为未来即时护理测试平台的基础。
