Cho H J, Bohac J G
Can J Comp Med. 1985 Apr;49(2):189-94.
An enzyme-linked immunosorbent assay was developed to detect bovine serum antibody to infectious bovine rhinotracheitis virus. The specificity of this assay in 304 bovine sera, collected from an infectious bovine rhinotracheitis virus-free herd, was 100%; in sera from 62 cattle inoculated with an intranasal vaccine, its diagnostic sensitivity was 27.4% at one month and 100% at six months, postvaccination. In 303 bovine sera with standard serum neutralizing antibody titers of greater than or equal to 1:2 it showed 100% sensitivity; and in 463 random diagnostic samples, comparative tests indicated that enzyme-linked immunosorbent assay detected more seropositive animals (61.6%) than the standard serum neutralizing test (49.9%). The enzyme-linked immunosorbent assay method was considered to be technically superior as a routine diagnostic test for the detection of infectious bovine rhinotracheitis viral antibody in bovine sera.
开发了一种酶联免疫吸附测定法来检测牛血清中针对牛传染性鼻气管炎病毒的抗体。该测定法在从无牛传染性鼻气管炎病毒的牛群中采集的304份牛血清中的特异性为100%；在接种鼻内疫苗的62头牛的血清中，接种疫苗后1个月其诊断敏感性为27.4%，6个月时为100%。在303份标准血清中和抗体效价大于或等于1:2的牛血清中，其敏感性为100%；在463份随机诊断样本中，对比试验表明酶联免疫吸附测定法检测到的血清阳性动物（61.6%）比标准血清中和试验（49.9%）更多。酶联免疫吸附测定法被认为作为检测牛血清中牛传染性鼻气管炎病毒抗体的常规诊断试验在技术上更具优势。
