扫描电化学显微镜在生物样品中的应用。

Lee C, Kwak J, Bard A J

Department of Chemistry, University of Texas, Austin 78712.

Proc Natl Acad Sci U S A. 1990 Mar;87(5):1740-3. doi: 10.1073/pnas.87.5.1740.

The scanning electrochemical microscope can be used in the feedback mode in two-dimensional scans over biological substrates to obtain topographic information at the micrometer level. In this mode, the effect of distance between a substrate (either conductive or insulating) and a scanning ultramicroelectrode tip on the electrolytic current flowing at the tip is recorded as a function of the tip x-y position. Scans of the upper surface of a grass leaf and the lower surface of a Ligustrum sinensis leaf (which show open stomata structures) immersed in aqueous solution are shown. Scans of the upper surface of an elodea leaf in the dark and under irradiation, where the tip reaction is the reduction of oxygen produced by photosynthesis, demonstrate the possibility of obtaining information about the distribution of reaction sites on the substrate surface.

扫描电化学显微镜可在生物基质上进行二维扫描时以反馈模式使用，以获取微米级别的地形信息。在这种模式下，记录底物（导电或绝缘）与扫描超微电极尖端之间的距离对尖端处流动的电解电流的影响，该影响是作为尖端x - y位置的函数。展示了浸入水溶液中的草叶上表面和女贞叶下表面（显示开放气孔结构）的扫描结果。对黑暗中和光照下的伊乐藻叶上表面进行扫描，其中尖端反应是光合作用产生的氧气的还原，这证明了获取有关底物表面反应位点分布信息的可能性。