ZhiYong Wang, Fei Song, LianJu Xu, Yingkai Liu, Chun Qing, Shuliang Lu, XiQiao Wang
Shanghai Jiaotong University, Shanghai, China.
Int J Low Extrem Wounds. 2012 Dec;11(4):271-6. doi: 10.1177/1534734612463698. Epub 2012 Oct 21.
This study aims to investigate the effect of Endostar injection on the rabbit ear hypertrophic scar formation and expand the use of Endostar. The rabbit ear hypertrophic scar models were established 4 weeks postoperation and were treated with Endostar injection; the control group was injected with saline, once a week, 3 times totally. At the seventh week, the scar tissue was harvested and processed with hematoxylin and eosin (HE) staining and CD34 immunohistochemistry and cell apoptosis assay. In addition, the endothelial cell was cultured and seeded on Martrigel with different concentrations of Endostar to observe the vessel tube formation. The results showed that the volume of the hypertrophic scar with Endostar injection was greatly reduced compared with what was seen in the control group; meanwhile, HE staining showed that the cell number decreased and collagen density became looser. In addition, the CD34 staining indicated that microvessel formation in the study group also decreased and cell apoptosis increased. In vitro, the addition of Endostar could reduce vessel tube formation in a dose-dependent manner. In conclusion, the Endostar is effective for hypertrophic scar inhibition and could be a potential tool to treat scars.
本研究旨在探讨恩度注射液对兔耳增生性瘢痕形成的影响，并拓展恩度的应用。兔耳增生性瘢痕模型于术后4周建立，给予恩度注射液治疗；对照组注射生理盐水，每周1次，共3次。第7周时，采集瘢痕组织，进行苏木精-伊红（HE）染色、CD34免疫组化及细胞凋亡检测。此外，培养内皮细胞，接种于含不同浓度恩度的基质胶上，观察血管生成情况。结果显示，与对照组相比，注射恩度的增生性瘢痕体积明显减小；同时，HE染色显示细胞数量减少，胶原密度变疏松。此外，CD34染色表明研究组微血管形成也减少，细胞凋亡增加。在体外，添加恩度可呈剂量依赖性减少血管生成。综上所述，恩度对增生性瘢痕具有抑制作用，可能是治疗瘢痕的一种潜在手段。
