Fang Quan, Huang Chunlan, You Chuangang, Ma Shaolin
First Affiliated Hospital of Xinjiang Medical University, Wulumuqi, China.
First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, China.
Int J Low Extrem Wounds. 2015 Dec;14(4):343-52. doi: 10.1177/1534734615598064. Epub 2015 Aug 27.
The purpose of this article is to investigate the effect of Opuntia stricta H (Cactaceae) extract on suppression of hypertrophic scar on ventral surface wounds of rabbit ears. Full thickness skin defection was established in a rabbit ear to simulate hypertrophic scar. Opuntia extract was sprayed on the wounds in the experimental group, and normal saline was used in the control group. After the wounds healed with scar formation, the hypertrophic scar tissue was harvested on days 22, 39, and 54 for histological analysis. The expression of type I and type III collagen and matrix metalloproteinase-1 (MMP-1) were evaluated by immunohistochemistry and real-time quantitative polymerase chain reaction. The results indicated that the scar of the control group is more prominent compared with the opuntia extract group. The expression of type I collagen in the opuntia extract group was lower than the control group, while type III collagen in opuntia extract group gradually increased and exceeded control group. The expression of MMP-1 decreased in the opuntia extract group, while the control group increased over time, but the amount of MMP-1 was much higher than that in the control group on day 22. In conclusion, opuntia extract reduces hypertrophic scar formation by means of type I collagen inhibition, and increasing type III collagen and MMP-1.T he novel application of opuntia extract may lead to innovative and effective antiscarring therapies.
本文旨在研究仙人掌提取物对兔耳腹侧创面增生性瘢痕的抑制作用。在兔耳上制造全层皮肤缺损以模拟增生性瘢痕。实验组在伤口上喷洒仙人掌提取物，对照组使用生理盐水。伤口愈合形成瘢痕后，在第22、39和54天采集增生性瘢痕组织进行组织学分析。通过免疫组织化学和实时定量聚合酶链反应评估I型和III型胶原蛋白以及基质金属蛋白酶-1（MMP-1）的表达。结果表明，与仙人掌提取物组相比，对照组的瘢痕更明显。仙人掌提取物组I型胶原蛋白的表达低于对照组，而III型胶原蛋白在仙人掌提取物组中逐渐增加并超过对照组。仙人掌提取物组中MMP-1的表达降低，而对照组随时间增加，但在第22天时MMP-1的量远高于对照组。总之，仙人掌提取物通过抑制I型胶原蛋白、增加III型胶原蛋白和MMP-1来减少增生性瘢痕的形成。仙人掌提取物的新应用可能会带来创新且有效的抗瘢痕治疗方法。
