Chacko Anita R, Zwart Peter H, Read Randy J, Dodson Eleanor J, Rao C D, Suguna Kaza
Molecular Biophysics Unit, Indian Institute of Science, C. V. Raman Avenue, Bangalore, Karnataka 560 012, India.
Acta Crystallogr D Biol Crystallogr. 2012 Nov;68(Pt 11):1541-8. doi: 10.1107/S090744491203836X. Epub 2012 Oct 18.
The crystal structure of the region spanning residues 95-146 of the rotavirus nonstructural protein NSP4 from the asymptomatic human strain ST3 was determined at a resolution of 2.5 Å. Severe diffraction anisotropy, rotational pseudosymmetry and twinning complicated the refinement of this structure. A systematic explanation confirming the crystal pathologies and describing how the structure was successfully refined is given in this report.
测定了来自无症状人类毒株ST3的轮状病毒非结构蛋白NSP4中95 - 146位残基区域的晶体结构，分辨率为2.5 Å。严重的衍射各向异性、旋转假对称性和孪晶使该结构的精修变得复杂。本报告给出了一个系统的解释，证实了晶体的异常情况，并描述了该结构是如何成功精修的。
