Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Department of Environmental Science, Zhejiang University, Hangzhou, China.
Arch Microbiol. 2012 Dec;194(12):1043-8. doi: 10.1007/s00203-012-0849-3. Epub 2012 Oct 23.
The presence and diversity of acyl homoserine lactone (AHL)-producers in an urban river biofilm were investigated during 60-day biofilm formation. AHL biosensors detected the presence of AHL-producers in 1-60-day river biofilms. Screening for AHL-producers resulted in 17 Aeromonas spp., 3 Pseudomonas spp., 3 Ensifer spp., and 1 Acinetobacter sp. Among these isolates, six of them were closely related to Acinetobacter tjernbergiae, Aeromonas allosaccharophila, Aeromonas aquariorum, Aeromonas jandaei, Pseudomonas panipatensis, and Ensifer adhaerens and represented novel AHL-producing species. Thin layer chromatography revealed that C4-homoserine lactone was prevailing in Aeromonas spp., whereas C6- and C8-homoserine lactones and their derivatives were prevailing in other strains. Using degenerate primers, novel AHL synthetase genes from the three Ensifer spp. were successfully amplified. This study reports for the first time the diversity of AHL-producers from a river biofilm and the variety of novel AHL synthetase genes in Ensifer group.
在 60 天的生物膜形成过程中，研究了城市河流生物膜中酰基高丝氨酸内酯 (AHL) 产生菌的存在和多样性。AHL 生物传感器检测到 1-60 天河流生物膜中 AHL 产生菌的存在。对 AHL 产生菌的筛选得到了 17 种气单胞菌、3 种假单胞菌、3 种土壤杆菌和 1 种不动杆菌。在这些分离物中，有 6 种与阿克曼氏菌 tjernbergiae、嗜糖气单胞菌、杀鲑气单胞菌、杰氏气单胞菌、泛菌 panipatensis 和粘质沙雷氏菌密切相关，代表了新型 AHL 产生菌。薄层层析表明，C4-高丝氨酸内酯在气单胞菌中占优势，而 C6-和 C8-高丝氨酸内酯及其衍生物在其他菌株中占优势。使用简并引物，成功地从 3 种土壤杆菌中扩增出了新型 AHL 合成酶基因。本研究首次报道了河流生物膜中 AHL 产生菌的多样性和土壤杆菌属中新型 AHL 合成酶基因的多样性。
