Zeng Yanhua, Wang Yanli, Yu Zhiliang, Huang Yili
Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang UniversityHangzhou, China.
College of Biotechnology and Bioengineering, Zhejiang University of TechnologyHangzhou, China.
Front Microbiol. 2017 Jun 28;8:1160. doi: 10.3389/fmicb.2017.01160. eCollection 2017.
It is known that some bacteria, especially members of the family , have multiple -acyl homoserine lactones (AHL) synthase genes and produce multiple AHL signals. However, how bacteria selectively utilize these multiple genes and signals to cope with changing environments is poorly understood. is an important microorganism in terms of biotechnology, ecology and evolutionary. In this study, we investigated the AHL-based QS system of X097 and its response to different lifestyles or nutrients. Draft genome sequence data indicated that X097 harbored three distinct AHL synthase genes () and seven homologs, which was different from other strains. expression indicated that plasmid-encoded and directed production of multiple AHLs, while chromosome-encoded only directed production of C14-HSL. Predicted three dimensional structure of EnsI3 was quite different from that of EnsI1 and EnsI2. X097 produced different AHL profiles in Luria-Bertani (LB) and NFB medium, under biofilm and planktonic lifestyle, respectively. Notably, expression of and but not is hypersensitive to different lifestyles and nutrients. The hypersensitive response of plasmid-encoded AHL synthase genes to different culture conditions may shed a light on the phylogenetic development of AHL synthase genes in family.
已知一些细菌，尤其是该家族的成员，具有多个酰基高丝氨酸内酯（AHL）合酶基因并产生多种AHL信号。然而，细菌如何选择性地利用这些多个基因和信号来应对不断变化的环境，目前还知之甚少。就生物技术、生态学和进化而言，是一种重要的微生物。在本研究中，我们研究了X097基于AHL的群体感应系统及其对不同生活方式或营养物质的反应。基因组草图序列数据表明，X097含有三个不同的AHL合酶基因（）和七个同源物，这与其他菌株不同。表达表明，质粒编码的和指导多种AHL的产生，而染色体编码的仅指导C14-HSL的产生。预测的EnsI3三维结构与EnsI1和EnsI2的三维结构有很大不同。X097分别在生物膜和浮游生活方式下，于Luria-Bertani（LB）和NFB培养基中产生不同的AHL谱。值得注意的是，和的表达对不同生活方式和营养物质敏感，而的表达则不然。质粒编码的AHL合酶基因对不同培养条件的敏感反应可能为该家族中AHL合酶基因的系统发育发展提供线索。
