Department of Food and Nutrition, Kyungnam University, Changwon, Korea.
Poult Sci. 2012 Nov;91(11):2747-54. doi: 10.3382/ps.2012-02150.
Food protein-derived peptides are important components for nutraceuticals, with many biological functions as well as substantial nutritional benefits. The aim of this study was to investigate the antioxidant effects of ovotransferrin (OTF) derived from egg white and its hydrolysates (OH) prepared by hydrolyzing either with acid or enzymes (protamex, alkalase, trypsin, neutrase, flavorzyme, maxazyme, collupulin, protex, promod 278, and α-chymotrypsin). All OH showed approximately 3.2 to 13.5 times higher superoxide anion scavenging activity than OTF, with the maximum activity found in the OH-protamex. Similar results were obtained for oxygen radical absorbance capacity assay, with the highest value in OH-α-chymotrypsin [1.6 μM trolox equivalents (TE)] and the lowest value in OTF (0.2 μM TE). However, OTF showed the most powerful 2,2-diphenyl-2-picrylhydrazyl radical scavenging activity, which reached 78.2% after 36 h of reaction. Both OTF and OH showed protective effects against the oxidative stress-induced DNA damages in human leukocytes. Overall, OTF possessed antioxidant abilities and hydrolyzation of OTF with acid or enzymes improved these abilities.
食物蛋白衍生肽是营养保健品的重要组成部分，具有多种生物功能和显著的营养价值。本研究旨在探讨卵转铁蛋白（OTF）及其由酸或酶（胃蛋白酶、碱性酶、胰蛋白酶、中性蛋白酶、风味蛋白酶、Maxazyme、Collupulin、Protex、Promod 278 和 α-糜蛋白酶）水解制备的水解产物（OH）的抗氧化作用。所有 OH 的超氧阴离子清除活性均比 OTF 高约 3.2 至 13.5 倍，其中 OH-胃蛋白酶的活性最高。氧自由基吸收能力测定也得到了类似的结果，OH-α-糜蛋白酶的最高值为 1.6 μM 生育酚当量（TE），OTF 的最低值为 0.2 μM TE。然而，OTF 表现出最强的 2,2-二苯基-2-苦肼基自由基清除活性，在反应 36 小时后达到 78.2%。OTF 和 OH 均对人白细胞的氧化应激诱导的 DNA 损伤具有保护作用。总的来说，OTF 具有抗氧化能力，酸或酶水解 OTF 可以提高其抗氧化能力。
