Department of Animal Science, Iowa State University, Ames, Iowa 50011.
Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Korea.
Poult Sci. 2017 Jun 1;96(6):1982-1987. doi: 10.3382/ps/pew459.
Peptides released from egg proteins via enzymatic hydrolysis show various bioactivities such as antimicrobial, antioxidant, antihypertensive, and immunomodulatory properties. The objective of this study was to investigate the cytotoxic and Angiotensin Converting Enzyme (ACE)-inhibitory activities of ovotransferrin and its promod 278P enzyme hydrolysate. Ovotransferrin from egg white was hydrolyzed using promod 278P at 45°C for 3 hours. Using the MTT assay, the cytotoxicity of ovotransferrin and promod 278P hydrolysate of ovotransferrin were evaluated in human cancer cell lines of various tissue origins. The ACE-inhibitory activity was determined using the cleavage of a chromogenic substrate -Hip-His-Leu. The promod 278P hydrolysate of ovotransferrin showed a potent cytotoxicity (>90%) at 20 mg/mL in all cancer cell lines tested, but ovotransferrin did not. The IC50 value of the promod 278P hydrolysate of ovotransferrin against 5 different cancer cells were 10.05 ± 1.55, 3.45 ± 0.94, 4.43 ± 1.87, 4.92 ± 0.63, and 10.43 ± 3.91 mg/mL for MCF-7, HeLa, HepG2, HT-29, and LoVo cells, respectively. The promod 278P hydrolysate of ovotransferrin showed a strong ACE-inhibiting activity: at 10 mg/mL level, the hydrolysate showed 76.82 ± 1.28% inhibition to ACE-inhibitory activity, and 73.33 ± 2.56%, 56.85 ± 1.84%, 50.32 ± 3.71%, 17.30 ± 0.13%, and 4.52 ± 6.83% inhibitory activity at 5, 2.5, 1.25, 0.625, and 0.3125 mg/mL level, respectively. The IC50 value of the promod 278P hydrolysate of ovotransferrin was 1.53 ± 0.20 mg/mL. However, ovotransferrin did not show any inhibitory effect to angiotensin-converting enzyme activity. This result indicated that the promod 278P hydrolysate of ovotransferrin has a great potential as an anticancer and antihypertension agent for humans, but the information on the peptides responsible for the functional activities is not available yet.
通过酶解从卵蛋白中释放的肽显示出各种生物活性，如抗菌、抗氧化、降压和免疫调节特性。本研究旨在研究卵转铁蛋白及其 promod 278P 酶解物的细胞毒性和血管紧张素转化酶 (ACE) 抑制活性。卵转铁蛋白来自蛋清，在 45°C 下用 promod 278P 水解 3 小时。使用 MTT 测定法，评估了卵转铁蛋白和卵转铁蛋白 promod 278P 水解产物在各种组织来源的人癌细胞系中的细胞毒性。ACE 抑制活性通过裂解发色底物 Hip-His-Leu 来确定。卵转铁蛋白 promod 278P 水解产物在所有测试的癌细胞系中在 20mg/mL 时表现出很强的细胞毒性 (>90%)，但卵转铁蛋白没有。卵转铁蛋白 promod 278P 水解产物对 5 种不同癌细胞的 IC50 值分别为 10.05±1.55、3.45±0.94、4.43±1.87、4.92±0.63 和 10.43±3.91mg/mL MCF-7、HeLa、HepG2、HT-29 和 LoVo 细胞。卵转铁蛋白 promod 278P 水解产物具有很强的 ACE 抑制活性：在 10mg/mL 水平下，水解产物对 ACE 抑制活性的抑制率为 76.82±1.28%，在 5、2.5、1.25、0.625 和 0.3125mg/mL 水平下，抑制率分别为 73.33±2.56%、56.85±1.84%、50.32±3.71%、17.30±0.13%和 4.52±6.83%。卵转铁蛋白 promod 278P 水解产物的 IC50 值为 1.53±0.20mg/mL。然而，卵转铁蛋白对血管紧张素转化酶活性没有显示出任何抑制作用。该结果表明，卵转铁蛋白 promod 278P 水解产物具有作为人类抗癌和降压剂的巨大潜力，但负责功能活性的肽的信息尚不可用。
