蛋白酶催化的有机相不对称 Mannich 反应。
Protease-catalysed direct asymmetric Mannich reaction in organic solvent.
机构信息
School of Chemistry and Chemical Engineering, Southwest University , Chongqing, 400715, P. R. China.
出版信息
Sci Rep. 2012;2:761. doi: 10.1038/srep00761. Epub 2012 Oct 23.
Abstract
We reported the first enzyme-catalysed, direct, three-component asymmetric Mannich reaction using protease type XIV from Streptomyces griseus (SGP) in acetonitrile. Yields of up to 92% with enantioselectivities of up to 88% e.e. and diastereoselectivities of up to 92:8 (syn:anti) were achieved under the optimised conditions. This enzyme's catalytic promiscuity expands the application of this biocatalyst and provides a potential alternative method for asymmetric Mannich reactions.
摘要
我们报道了首例使用灰色链霉菌（Streptomyces griseus）蛋白酶 XIV 在乙腈中进行的酶催化、直接、三元不对称曼尼希反应。在优化条件下，最高产率可达 92%，对映选择性最高可达 88%ee，非对映选择性最高可达 92:8（syn:anti）。该酶的催化混杂性扩展了该生物催化剂的应用，并为不对称曼尼希反应提供了一种潜在的替代方法。
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