Institute of Animal Sciences and Veterinary, Hainan Academy of Agricultural Sciences, Haikou, Hainan, 571100, People’s Republic of China.
Mol Biol Rep. 2012 May;39(5):6329-35. doi: 10.1007/s11033-012-1454-x.
PCR-RFLP was used to analyze the polymorphisms of MC4R, LEP, H-FABP genes in a swine breed composite (DIV2) and 4 swine breeds (Yorkshire, Landrace, Meishan, Bamei). The association study of these polymorphisms with several economic traits was carried out on a DIV2 population. The results obtained showed that MC4R/TaqI genotype had an effect for average backfat thickness (P < 0.05) and lean meat percentage (P < 0.05). At locus LEP/HinfI animals of AA genotype had lower test daily gain than that of BB (P < 0.01) or AB genotype (P < 0.05). At the H-FABP/HaeIII locus lean meat percentage of the individuals with genotype DD were higher than that with genotype dd (P < 0.05). Linkage disequilibrium analysis among MC4R, LEP and H-FABP revealed that these genes were independent. This represented two or more genes that could be combined together within one genotype in order to facilitate breeding for objective traits. In addition, a method allowing simultaneous detection of fragments of MC4R and LEP gene was developed.
聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）分析了猪品种复合群体（DIV2）和 4 个猪品种（约克夏、长白、梅山、巴马）中 MC4R、LEP、H-FABP 基因的多态性。对这些多态性与几个经济性状的关联研究是在 DIV2 群体中进行的。结果表明，MC4R/TaqI 基因型对平均背膘厚（P < 0.05）和瘦肉率（P < 0.05）有影响。在 LEP/HinfI 基因座上，AA 基因型的动物比 BB（P < 0.01）或 AB 基因型（P < 0.05）的日增重低。在 H-FABP/HaeIII 基因座上，基因型 DD 的个体的瘦肉率高于基因型 dd（P < 0.05）。MC4R、LEP 和 H-FABP 之间的连锁不平衡分析表明，这些基因是独立的。这代表了两个或更多的基因可以在一个基因型中组合在一起，以便于为目标性状进行选育。此外，还开发了一种可以同时检测 MC4R 和 LEP 基因片段的方法。
