Mencher D, Shouval D, Reshef L
Eur J Biochem. 1979 Dec 17;102(2):489-95. doi: 10.1111/j.1432-1033.1979.tb04264.x.
Injection of streptozotocin in utero to fetuses elicited a premature appearance of cytosolic hepatic activity of phosphoenol pyruvate carboxykinase. This was due to a precocious initiation of the synthesis of the enzyme. The streptozotocin-induced appearance of enzyme activity was not mediated by adenosine 3':5'-monophosphate since the concentration of the cyclic nucleotide in the liver was unaffected by the antibiotic, the administration of dibutyryladenosine 3':5'-monophosphate to streptozotocin-treated fetuses elicited an additive increase in enzyme activity, and insulin administration in utero repressed the streptozotocin effect while the effect due to dibutyryladenosine 3':5'-monophosphate was not inhibited by simultaneous insulin injection. Streptozotocin treatment also caused a small but consistent retardation of fetal growth and a marked reduction of liver wet weight. Histological analysis of the liver demonstrated a premature loss of some hematopoietic elements, while hepatocytes appeared normal. Hepatic protein synthesis was unaffected by the streptozotocin treatment. Streptozotocin treatment had no effect on fetal renal phosphoenol pyruvate carboxykinase activity or kidney wet weight.
给胎儿子宫内注射链脲佐菌素会引发磷酸烯醇式丙酮酸羧激酶胞质肝活性过早出现。这是由于该酶合成的过早启动。链脲佐菌素诱导的酶活性出现并非由3':5'-环磷酸腺苷介导，因为肝脏中该环核苷酸的浓度不受抗生素影响，给链脲佐菌素处理的胎儿注射二丁酰环磷腺苷会使酶活性出现累加性增加，而子宫内注射胰岛素可抑制链脲佐菌素的作用，同时注射胰岛素时二丁酰环磷腺苷所致的作用未被抑制。链脲佐菌素处理还导致胎儿生长出现轻微但持续的迟缓以及肝脏湿重显著降低。肝脏组织学分析显示一些造血成分过早丧失，而肝细胞外观正常。链脲佐菌素处理对肝脏蛋白质合成无影响。链脲佐菌素处理对胎儿肾磷酸烯醇式丙酮酸羧激酶活性或肾脏湿重无影响。
