College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Microsurgery. 2012 Nov;32(8):642-8. doi: 10.1002/micr.22046. Epub 2012 Oct 25.
Ischemia-reperfusion injury (IRI) is usually the key and often plays an irreversible role to induce flap compromise in microvascular tissue transfers. This article aims to profile the expression of micro-RNAs (miRs) in free flap surgeries following IRI.
The miRs expression profiling was initially surveyed in rat epigastric flap vessels using Agilent 350-Microarrayed miRs after IRI, and then quantified by real-time reverse transcription polymerase chain reaction in flap vessels and tissues (n = 5) at three intervals: before induction of ischemia (normoxia without IRI, sham), 2 and 72 hours after reperfusion following 2 hours of ischemia. Furthermore, for seven patients with free anterolateral thigh flap reconstruction, the miRs expression patterns in these flaps before induction of ischemia (normoxia), at 2 and 72 hours after reperfusion following an ischemic interval were investigated.
Four miRs (miR-96, miR-193-3p, miR-210, and miR-21) of 350 tested rat miRs were found to be positively significant. In rat flap vessels, the upregulation of these miRs at 72-hour reperfusion was statistically significant. These patterns were not noted in rat flap tissues, except for miR-96. However, there seemed to be no significant difference in human flap vessels between normoxia and 2-hour reperfusion following ischemia. In human flap tissue, significant upregulation of miR-193-3p, miR-210, and miR-21 was detected at 72-hour perfusion.
Our findings show some changes of four upregulated miRs in our model of IRI. We suggest that further investigation is needed to determine the role of miRs in IRI of microsurgical reconstruction.
缺血再灌注损伤（IRI）通常是导致微血管组织移植皮瓣失代偿的关键因素，且常常起着不可逆转的作用。本文旨在分析 IRI 后游离皮瓣手术中 microRNAs（miRs）的表达情况。
首先，采用 Agilent 350-Microarrayed miRs 对大鼠腹壁皮瓣血管中的 miRs 表达谱进行了初步调查，然后通过实时逆转录聚合酶链反应（real-time RT-PCR）在皮瓣血管和组织（n=5）中对其进行了定量检测，分别在三个时间点进行：缺血前（无 IRI 缺氧，假手术）、缺血 2 小时再灌注后 2 小时和 72 小时。此外，对 7 例游离股前外侧皮瓣重建患者，在缺血前（正常氧合，无 IRI）、再灌注后 2 小时和 72 小时，检测这些皮瓣中 miRs 的表达模式。
在 350 种测试大鼠 miRs 中，有 4 种 miRs（miR-96、miR-193-3p、miR-210 和 miR-21）呈阳性显著。在大鼠皮瓣血管中，这些 miRs 在 72 小时再灌注时的上调具有统计学意义。在大鼠皮瓣组织中，除了 miR-96 外，并未发现这些 miR 的表达模式。然而，在人类皮瓣血管中，在缺血后再灌注 2 小时与正常氧合之间似乎没有明显差异。在人类皮瓣组织中，在灌注 72 小时时检测到 miR-193-3p、miR-210 和 miR-21 的显著上调。
我们的研究结果显示，在我们的 IRI 模型中，四种上调的 miR 存在一些变化。我们建议需要进一步研究以确定 miRs 在显微重建中 IRI 的作用。
