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局灶性脑缺血损伤后大鼠 microRNAs 的鉴定和功能分析。

Identification and functional analysis of microRNAs in rats following focal cerebral ischemia injury.

机构信息

School of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui 230012, P.R. China.

School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 211198, P.R. China.

出版信息

Mol Med Rep. 2019 May;19(5):4175-4184. doi: 10.3892/mmr.2019.10073. Epub 2019 Mar 21.

DOI:10.3892/mmr.2019.10073
PMID:30896823
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6471137/
Abstract

MicroRNA sequencing (miRNA‑seq) was performed in the present study to investigate miRNA expression profiles in infarcted brain areas following focal cerebral ischemia induced by middle cerebral artery occlusion in rats. In total, 20 miRNAs were identified to be upregulated and 17 to be downregulated in the infarct area. The expression levels of six differentially expressed miRNAs (DEmiRs), miR‑211‑5p, miR‑183‑5p, miR‑10b‑3p, miR‑182, miR‑217‑5p and miR‑96‑5p, were examined by reverse transcription‑​quantitative polymerase chain reaction. Subsequently, a miRNA‑mRNA network was constructed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to investigate the functions of the mRNAs targeted by these DEmiRs. The present study aimed to investigate the association between miRNAs and cerebral ischemia to provide potential insight into the molecular mechanisms underlying ischemic stroke.

摘要

本研究通过对大鼠大脑中动脉闭塞诱导的局灶性脑缺血后梗死区域进行 microRNA 测序（miRNA-seq），以研究 miRNA 的表达谱。总共鉴定出 20 个在梗死区域上调和 17 个下调的 miRNA。通过逆转录-定量聚合酶链反应（RT-qPCR）检测了六个差异表达 miRNA（DEmiRs）miR-211-5p、miR-183-5p、miR-10b-3p、miR-182、miR-217-5p 和 miR-96-5p 的表达水平。随后，构建了 miRNA-mRNA 网络。通过基因本体论和京都基因与基因组百科全书分析，研究了这些 DEmiRs 靶向的 mRNAs 的功能。本研究旨在探讨 miRNA 与脑缺血之间的关系，为缺血性脑卒中的分子机制提供潜在的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/52d2f6e7afbd/MMR-19-05-4175-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/cb2cbc23ddfa/MMR-19-05-4175-g00.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/e56a02c0aed1/MMR-19-05-4175-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/1ead0e9a140e/MMR-19-05-4175-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/6d039c096e5e/MMR-19-05-4175-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/6d325ac77e2d/MMR-19-05-4175-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/52d2f6e7afbd/MMR-19-05-4175-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/cb2cbc23ddfa/MMR-19-05-4175-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/7d79a03c1ef3/MMR-19-05-4175-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/1caddfe3b0ba/MMR-19-05-4175-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/e56a02c0aed1/MMR-19-05-4175-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/1ead0e9a140e/MMR-19-05-4175-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/6d039c096e5e/MMR-19-05-4175-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/6d325ac77e2d/MMR-19-05-4175-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ca0/6471137/52d2f6e7afbd/MMR-19-05-4175-g07.jpg

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