Sztein J M, Farley J S, Mobraaten L E
The Jackson Laboratory, Bar Harbor, Maine 04609, USA.
Biol Reprod. 2000 Dec;63(6):1774-80. doi: 10.1095/biolreprod63.6.1774.
Sperm from C57BL/6J, DBA/2J, BALB/cJ, 129S3/SvImJ, and FVB/NJ inbred mice were cryopreserved in 3% skim milk/18% raffinose cryoprotectant solution. The post-thaw sperm from all strains were evaluated for their viability and fertility by comparing them against B6D2F1 sperm used as a control. The protocol used for freezing mouse sperm was effective in different strains, because the motility was decreased by 50% after cryopreservation similar to other mammalian sperm. However, the progressive motility and the fertility of each inbred strain were affected differently. The C57BL/6J, BALB/cJ, and 129S3/SvImJ strains were the most affected; their fertility (two-cell cleavage) decreased from 70%, 34%, and 84% when using freshly collected sperm to 6%, 12%, and 6% when using frozen/thawed sperm, respectively. Live newborns derived from frozen/thawed sperm were obtained from all strains in the study. These results corroborate the genetic variation among strains with regard to fertility and susceptibility to cryopreservation.
将C57BL/6J、DBA/2J、BALB/cJ、129S3/SvImJ和FVB/NJ近交系小鼠的精子保存在3%脱脂乳/18%棉子糖冷冻保护剂溶液中。通过与用作对照的B6D2F1精子比较，对所有品系解冻后的精子的活力和生育力进行评估。用于冷冻小鼠精子的方案在不同品系中均有效，因为冷冻保存后精子活力下降了50%，这与其他哺乳动物精子类似。然而，每个近交系的前向运动能力和生育力受到的影响有所不同。C57BL/6J、BALB/cJ和129S3/SvImJ品系受影响最大；它们的生育力（双细胞分裂）分别从使用新鲜采集精子时的70%、34%和84%降至使用冻融精子时的6%、12%和6%。本研究中所有品系均获得了来自冻融精子的活产新生仔。这些结果证实了品系间在生育力和对冷冻保存的敏感性方面存在遗传差异。
