开发一种针对 Trichosporon 种属的肽核酸探针，并通过原位杂交技术在福尔马林固定石蜡包埋（FFPE）切片中鉴定 Trichosporon 病。
Development of a peptide nucleic acid probe to Trichosporon species and identification of trichosporonosis by use of in situ hybridization in formalin-fixed and paraffin-embedded (FFPE) sections.
机构信息
Department of Surgical Pathology, Toho University School of Medicine, Tokyo, Japan.
出版信息
J Clin Microbiol. 2013 Jan;51(1):295-8. doi: 10.1128/JCM.02221-12. Epub 2012 Oct 24.
Abstract
In order to identify Trichosporon species in formalin-fixed and paraffin-embedded sections from which visual discrimination of non-glabrata Candida species is mostly ineffective but critical for the choice of antifungals, we tested the usefulness of a newly designed peptide nucleic acid probe (PNA) for in situ hybridization (ISH). Results confirmed the usefulness of ISH with our PNA probe in identifying Trichosporon species from Candida albicans.
摘要
为了在福尔马林固定和石蜡包埋切片中鉴定出毛孢子菌属物种，而这些切片中，非光滑假丝酵母的肉眼鉴别效果大多不佳，但对抗真菌药物的选择至关重要，我们测试了一种新设计的肽核酸探针（PNA）用于原位杂交（ISH）的有用性。结果证实，我们的 PNA 探针用于ISH 可以识别出白色念珠菌中的毛孢子菌属物种。
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