Department of Surgical Pathology, Toho University School of Medicine, Tokyo, Japan.
J Clin Microbiol. 2013 Jan;51(1):295-8. doi: 10.1128/JCM.02221-12. Epub 2012 Oct 24.
In order to identify Trichosporon species in formalin-fixed and paraffin-embedded sections from which visual discrimination of non-glabrata Candida species is mostly ineffective but critical for the choice of antifungals, we tested the usefulness of a newly designed peptide nucleic acid probe (PNA) for in situ hybridization (ISH). Results confirmed the usefulness of ISH with our PNA probe in identifying Trichosporon species from Candida albicans.
为了在福尔马林固定和石蜡包埋切片中鉴定出毛孢子菌属物种,而这些切片中,非光滑假丝酵母的肉眼鉴别效果大多不佳,但对抗真菌药物的选择至关重要,我们测试了一种新设计的肽核酸探针(PNA)用于原位杂交(ISH)的有用性。结果证实,我们的 PNA 探针用于ISH 可以识别出白色念珠菌中的毛孢子菌属物种。