耐热突变株 Rhizobium sp. [Cajanus] 的生长、存活和共生特性研究。
Characterization of heat resistant mutant strains of Rhizobium sp. [Cajanus] for growth, survival and symbiotic properties.
机构信息
Department of Biotechnology, Chaudhary Devi Lal University, Sirsa, 125 055 Haryana India.
出版信息
Indian J Microbiol. 2007 Dec;47(4):329-35. doi: 10.1007/s12088-007-0060-4. Epub 2008 Jan 11.
Fourteen heat resistant mutant strains were isolated from a wild-type strain (PP201, Nod(+) Fix(+)) of Rhizobium sp. (Cajanus) by giving it a heat shock of 43°C. These mutant strains showed a greater increase in optical density (O.D.) and a higher viable cell count in both rhizospheric and non-rhizospheric soil at high temperature. Symbiotic studies showed that pigeon pea plants inoculated with a few mutant strains had ineffective nodules (Nod(+) Fix(-)) under controlled temperature (43°C) conditions, but under natural high temperature (40-45°C) conditions, the host plants infected with all the mutant strains showed higher total shoot nitrogen than the plants inoculated with the parent strain. Four mutant strains (HR-3, HR-6, HR-10 and HR-12) were found to be highly efficient for all the symbiotic parameters, and thus have the potential to be used as bioinoculants in the North-Western regions of India during the summer season.
从一株野生型根瘤菌（Cajanus）（PP201，Nod(+) Fix(+)）出发，通过 43°C 的热休克，分离出 14 株耐热突变株。这些突变株在高温下的根际和非根际土壤中，光密度（O.D.）的增加和活细胞数的增加更为显著。共生研究表明，在控制温度（43°C）条件下，接种少数突变株的羽扇豆植物会形成无效的根瘤（Nod(+) Fix(-)），但在自然高温（40-45°C）条件下，所有突变株感染的宿主植物的总茎氮含量均高于接种亲本菌株的植物。发现 4 株突变株（HR-3、HR-6、HR-10 和 HR-12）在所有共生参数方面均具有高效性，因此有可能在印度西北部的夏季作为生物接种剂使用。
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