Animal Biotechnology Lab. Regional Station, Indian Veterinary Research Institute, Palampur, 176 061 India.
Indian J Microbiol. 2008 Jun;48(2):216-27. doi: 10.1007/s12088-008-0027-0. Epub 2008 Jun 17.
Metagenomics- the application of the genomics technologies to nonculturable microbial communities, is coming of age. These approaches can be used for the screening and selection of nonculturable rumen microbiota for assessing their role in gastrointestinal (GI) nutrition, plant material fermentation and the health of the host. The technologies designed to access this wealth of genetic information through environmental nucleic acid extraction have provided a means of overcoming the limitations of culture-dependent microbial genetic exploitation. The molecular procedures and techniques will result in reliable insights into the GI microbial structure and activity of the livestock gut microbes in relation to functional interactions, temporal and spatial relationships among different microbial consortia and dietary ingredients. Future developments and applications of these methods promise to provide the first opportunity to link distribution and identity of rumen microbes in their natural habitats with their genetic potential and in situ activities.
宏基因组学——将基因组学技术应用于不可培养的微生物群落，正在走向成熟。这些方法可用于筛选和选择不可培养的瘤胃微生物群，以评估它们在胃肠道（GI）营养、植物物质发酵和宿主健康中的作用。这些旨在通过环境核酸提取来获取这些丰富遗传信息的技术，为克服依赖培养的微生物遗传利用的局限性提供了一种手段。分子程序和技术将为了解 GI 微生物结构和家畜肠道微生物的活性提供可靠的信息，包括功能相互作用、不同微生物群落之间的时空关系以及膳食成分。这些方法的未来发展和应用有望首次提供机会，将瘤胃微生物在其自然栖息地的分布和身份与其遗传潜力和原位活性联系起来。
