Singh Birbal, Gautam Sanjeev K, Verma Vinod, Kumar Manoj, Singh Bhupinder
Indian Veterinary Research Institute, Regional Station, Palampur 176 061, India.
Anaerobe. 2008 Jun;14(3):138-44. doi: 10.1016/j.anaerobe.2008.03.002. Epub 2008 Mar 26.
Microbial metagenomics---the applications of the genomics suit of technologies to nonculturable microorganisms, is coming of age. These approaches can be used for the screening and identification of nonculturable gastrointestinal (GI) microflora for assessing and exploiting them in nutrition and the health of the host. Advances in technologies designed to access this wealth of genetic information through environmental nucleic acids extraction and analysis have provided the means of overcoming the limitations of conventional culture-dependent microbial genetic exploitation. The molecular techniques and bioinformatics tools will result in reliable insights into the animals' GI microbial structure and activity of the livestock gut microbes in relation to functional interactions, temporal and spatial relationships among different microbial consortia and dietary ingredients. Further developments and applications of these methods promise to provide the opportunity to link distribution and identity of various GI microbes in their natural habitats, and explore their use for promoting livestock health and industrial development.
微生物宏基因组学——将基因组技术应用于不可培养微生物，正逐渐走向成熟。这些方法可用于筛选和鉴定不可培养的胃肠道微生物群落，以评估并在营养和宿主健康方面加以利用。通过环境核酸提取和分析来获取这一丰富遗传信息的技术进展，为克服传统依赖培养的微生物遗传开发的局限性提供了手段。分子技术和生物信息学工具将使人们可靠地洞察动物胃肠道微生物结构以及家畜肠道微生物在功能相互作用、不同微生物群落与膳食成分之间的时空关系方面的活性。这些方法的进一步发展和应用有望提供机会，将各种胃肠道微生物在其自然栖息地中的分布和特性联系起来，并探索其在促进家畜健康和产业发展方面的用途。
