假单胞菌属对 4-氯苯甲酸的脱卤作用。
Dehalogenation of 4 - Chlorobenzoic Acid by Pseudomonas isolates.
机构信息
Department of Microbiology, Punjab Agricultural University, Ludhiana, 141 004 India.
出版信息
Indian J Microbiol. 2007 Jun;47(2):139-43. doi: 10.1007/s12088-007-0027-5. Epub 2007 Jul 8.
Twenty three bacterial isolates either pure or consortium were initially screened on the basis of their ability to degrade as well as dechlorinate 4 - chlorobenzoic acid (4-CBA). Based on comparative growth response, three pure isolates Pseudomonas putida GVS-4, Pseudomonas aeruginosa GVS-18 and Pseudomonas aeruginosa GWS-19 and a consortium SW-2 was finally selected for further studies. The enzyme studies performed with cell free extracts revealed that dehalogenase activity was substrate specific with maximum activity at 300 μgml(-1) substrate concentration. Catechol 1,2 dioxygenase activity was found to be present in cell free extracts suggesting that 4 - chlorobenzoic acid (4-CBA) is catabolized by ortho-ring cleavage pathway. The dehalogenase enzyme profile showed single enzyme band in case of GVS-4 (Rm 0.76), GVS-18 (Rm 0.84), GWS -19 (Rm 0.85) and two bands in SW-2 (Rm 0.71 & 0.10).
最初，根据降解和脱氯 4-氯苯甲酸（4-CBA）的能力，对 23 种细菌分离物（纯培养物或混合培养物）进行了筛选。基于比较生长反应，最终选择了 3 种纯培养物（恶臭假单胞菌 GVS-4、铜绿假单胞菌 GVS-18 和铜绿假单胞菌 GWS-19）和 1 种混合培养物（SW-2）进行进一步研究。用无细胞提取物进行的酶研究表明，脱卤酶活性具有底物特异性，在 300μgml(-1)底物浓度下具有最大活性。无细胞提取物中存在儿茶酚 1,2-双加氧酶活性，表明 4-氯苯甲酸（4-CBA）通过邻环裂解途径进行代谢。脱卤酶酶谱显示 GVS-4（Rm 0.76）、GVS-18（Rm 0.84）、GWS-19（Rm 0.85）只有单酶带，而 SW-2 有两条带（Rm 0.71 和 0.10）。
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