优化黑曲霉新型凝集素的培养条件并对其进行特性分析。
Optimization of culture conditions and characterization of a new lectin from Aspergillus niger.
机构信息
Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, 147 002 Punjab India.
出版信息
Indian J Microbiol. 2009 Sep;49(3):219-22. doi: 10.1007/s12088-009-0041-x. Epub 2009 Jun 30.
Lectin activity was determined on solidified medium containing agar and in broth cultures of Aspergillus niger. The fungus was found to express 16 times higher activity in broth cultures, when grown in a medium adjusted to pH 5.5 at 30°C under stationary condition. Lectin activity was found to be expressed by 6-day-old mycelial cultures with maximum activity being expressed on 9th day of incubation. The crude lectin (total titer 1280) was found to be precipitated at 50% saturation of ammonium sulphate with 2.4-fold purifi cation and 83% yield in the precipitate. The partially purifi ed lectin was found to agglutinate all human, rat, mice and pig erythrocytes. It was found to have a strong binding affinity to mucin, asialofetuin and inulin.
在含有琼脂的固化培养基和黑曲霉的肉汤培养物中测定了凝集素活性。当在 30°C 下以静止状态在 pH 5.5 调节的培养基中生长时，发现真菌在肉汤培养物中的活性高 16 倍。在 6 天龄的菌丝培养物中发现了凝集素活性，最大活性在孵育的第 9 天表达。粗凝集素（总效价 1280）在 50%饱和度的硫酸铵中沉淀，沉淀中得到 2.4 倍的纯化和 83%的产率。部分纯化的凝集素可凝集所有人类、大鼠、小鼠和猪的红细胞。它被发现对粘蛋白、脱唾液酸胎球蛋白和菊粉具有很强的结合亲和力。
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