来自再生大鼠肝脏的微粒体颗粒的蛋白质合成
Protein synthesis by microsomal particles from regenerating rat liver.
作者信息
Campbell P N, Lowe E, Serck-Hanssen G
出版信息
Biochem J. 1967 Apr;103(1):280-8. doi: 10.1042/bj1030280.
Abstract
- Washed microsome particles from regenerating liver were shown to incorporate [(14)C]leucine into protein more actively than similar preparations from normal liver. 2. The total incorporation in the preparations from regenerating liver increased linearly with the amount of protein incubated, whereas this was not so with preparations from normal liver. 3. The greater activity of regenerating-liver microsomes appeared to be associated with the bound polysomes. 4. The size distribution of polysomes obtained after removal of membrane with deoxycholate was the same in normal and regenerating liver. 5. In general the activity of polysome preparations from normal and regenerating liver was similar. 6. It is concluded that the greater activity of the particles in the microsome fraction from regenerating liver is to be attributed to the ribosomes bound to membrane and that their activity is controlled by factors present in the membrane.
摘要
- 结果显示，来自再生肝脏的洗涤微粒体颗粒比来自正常肝脏的类似制剂更积极地将[（14）C]亮氨酸掺入蛋白质中。2. 来自再生肝脏的制剂中的总掺入量随孵育蛋白质的量呈线性增加，而来自正常肝脏的制剂则不然。3. 再生肝脏微粒体的较高活性似乎与结合的多核糖体有关。4. 用脱氧胆酸盐去除膜后获得的多核糖体的大小分布在正常肝脏和再生肝脏中相同。5. 一般来说，来自正常肝脏和再生肝脏的多核糖体制剂的活性相似。6. 得出的结论是，再生肝脏微粒体部分颗粒的较高活性应归因于与膜结合的核糖体，并且它们的活性受膜中存在的因素控制。
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