Saleem M, Nicholls D M
Biochem J. 1979 Apr 15;180(1):51-8. doi: 10.1042/bj1800051.
Homogenates of hindleg muscle were obtained from control and dystrophic male hamsters, 30 and 190 days of age, and were used to prepare the postmicrosomal pH5-supernatant fraction. The activity of this fraction in the incorporation of [14C]phenylalanyl-tRNA into peptides was increased in the dystrophic-muscle preparations. No such increase was found in brain or liver preparations from dystrophic hamsters. The increased capacity for aminoacyl-tRNA binding that was observed in preparations from dystrophic animals is discussed.
从30日龄和190日龄的对照雄性仓鼠和营养不良雄性仓鼠中获取后肢肌肉匀浆，用于制备微粒体后pH5上清液组分。在营养不良肌肉制剂中，该组分将[14C]苯丙氨酰 - tRNA掺入肽中的活性增加。在营养不良仓鼠的脑或肝制剂中未发现这种增加。讨论了在营养不良动物制剂中观察到的氨酰 - tRNA结合能力增加的情况。
