Soloaga Rolando N, Carrion Natalia A, Margari Alejandra M, Giovanakis Marta, Sujemecki Alicia, Efron Ernesto, Pidone Juan C, Guelfand Liliana I, Mendez Aranibar Mariela
Laboratorio de Microbiología del Hospital Naval Pedro Mallo, Patricias Argentinas 351 (1405) Ciudad Autónoma de Buenos Aires, Argentina.
Rev Argent Microbiol. 2012 Jul-Sep;44(3):165-9.
A prospective observational study was conducted in two hospitals of Buenos Aires city (Argentina); 191 clinically significant monomicrobial gram-negative bloodstream infections were included in the study, which combined the Bact-Alert System Blood culture machine and the Vitek 2C System. Organism identification and susceptibility results directly from blood culture bottles were compared with those obtained from cards inoculated with a standardized bacterial suspension obtained following subculture on agar. By comparing the results obtained from pure cultures with those by the Vitek 2C System as reference method, the agreement between the reference method and the direct identification from positive blood cultures was 99 %. By antimicrobial susceptibility testing, the overall categorical accuracy was 99 % (0.22 %, very major errors, 0.17 %, major errors and 0.61 %, minor errors). One hundred and eight (56,8 %) bloodstream infections were treated empirically with adequate antibiotics. After the results obtained directly from the bottles were reported, antimicrobial therapy was changed in 116 (60.7 %) of the episodes.
在布宜诺斯艾利斯市（阿根廷）的两家医院进行了一项前瞻性观察研究；该研究纳入了191例具有临床意义的单一微生物革兰氏阴性血流感染病例，研究中使用了Bact-Alert系统血培养仪和Vitek 2C系统。将直接从血培养瓶获得的微生物鉴定和药敏结果与从接种了在琼脂上继代培养后获得的标准化细菌悬液的卡片上获得的结果进行比较。以Vitek 2C系统作为参考方法，将纯培养物获得的结果与之比较，参考方法与阳性血培养直接鉴定之间的一致性为99%。通过抗菌药敏试验，总体分类准确率为99%（极重大错误0.22%，重大错误0.17%，轻微错误0.61%）。108例（56.8%）血流感染接受了经验性充分抗生素治疗。在报告直接从血培养瓶获得的结果后，116例（60.7%）病例的抗菌治疗方案发生了改变。
