Cejas Daniela, Fernández Canigia Liliana, Quinteros Mirta, Giovanakis Marta, Vay Carlos, Lascialandare Silvana, Mutti Daniel, Pagniez Gastón, Almuzara Marisa, Gutkind Gabriel, Radice Marcela
Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 (1113) Ciudad Autónoma de Buenos Aires, Argentina.
Rev Argent Microbiol. 2012 Jul-Sep;44(3):182-6.
CMY-2 Β-lactamase is an important cause of Β-lactam resistance in Enterobacteriaceae and constitutes the most widespread pAmpC. Although CMY-2 has been previously recognized in our region, the real prevalence and epidemiology of this resistance marker was uncertain. During August-October 2009, we conducted a multicenter, prospective study to determine pAmpC prevalence and to characterize CMY-2 producing Escherichia coli associated plasmids. Plasmid-encoded AmpC prevalence was 0.9 % in enterobacteria in this period, being CMY-2 prevalent and to a lesser extent DHA. Molecular typing of CMY-2- producing Escherichia coli isolates showed several lineages. Moreover, replicon typing of cmy-2- containing plasmids displayed a broad diversity in Inc/cmy-2 links. Therefore, association of cmy-2 with specific transposon elements may be responsible for the spread of this resistance marker in Enterobacteriaceae.
CMY-2 β-内酰胺酶是肠杆菌科细菌对β-内酰胺类抗生素耐药的一个重要原因，也是分布最为广泛的pAmpC酶。尽管CMY-2此前在我们地区已被发现，但这种耐药标志物的实际流行情况和流行病学特征尚不确定。2009年8月至10月期间，我们开展了一项多中心前瞻性研究，以确定pAmpC酶的流行情况，并对产CMY-2的大肠杆菌相关质粒进行特征分析。在此期间，肠杆菌科细菌中质粒编码的AmpC酶流行率为0.9%，以CMY-2为主，DHA酶较少见。对产CMY-2的大肠杆菌分离株进行分子分型显示出多个谱系。此外，对携带cmy-2的质粒进行复制子分型显示，Inc/cmy-2连接存在广泛的多样性。因此，cmy-2与特定转座子元件的关联可能是这种耐药标志物在肠杆菌科细菌中传播的原因。
