Harmon S M, Kautter D A
J Assoc Off Anal Chem. 1979 Sep;62(5):1007-10.
The Colworth Stomacher Model 400 homogenizer was compared with the Waring Blendor for preparing food homogenates to be examined for Clostridium perfringens. Forty-eight samples representing 6 different food types were inoculated with C. perfringens and examined by the AOAC official first action method for enumeration of C. perfringens in foods. Identical paired specimens of each food type were blended with the 2 devices, and plate counts were made as specified in the official first action method. The effects of frozen storage on plate counts were determined by examining 24 food samples that had been stored for 3 days at -68 degrees C and homogenized both devices. Results of a statistical analysis of the experimental data indicated no significant difference overall (P greater than 0.05) in the plate counts of homogenates prepared with the Waring Blendor or the Stomacher 400, either before or after frozen storage of the food samples. However, the overall plate count average of the 48 samples was slightly higher with the Waring Blendor than with the Stomacher 400 homogenizer.
将科沃思400型组织捣碎机与韦林搅拌器进行比较,以制备用于检测产气荚膜梭菌的食品匀浆。选取代表6种不同食品类型的48个样本,接种产气荚膜梭菌,并按照美国官方分析化学师协会(AOAC)的首次行动方法对食品中的产气荚膜梭菌进行计数检测。每种食品类型的相同配对样本分别用这两种设备进行搅拌,并按照官方首次行动方法的规定进行平板计数。通过检测24个在-68摄氏度下储存3天的食品样本并用这两种设备进行匀浆处理,来确定冷冻储存对平板计数的影响。对实验数据的统计分析结果表明,无论是在食品样本冷冻储存之前还是之后,用韦林搅拌器或400型组织捣碎机制备的匀浆平板计数总体上没有显著差异(P大于0.05)。然而,48个样本的总体平板计数平均值,韦林搅拌器比400型组织捣碎机略高。