Harmon S M
J Assoc Off Anal Chem. 1976 May;59(3):606-12.
A collaborative study was conducted in 10 laboratories to evaluate the performance of a new method for the enumeration of vegetative cells of Clostridium perfringens in foods. Results obtained by the new method were compared with results from the official first action method, 46.049-46.053. Per cent recoveries of 4 C. perfringens strains from inoculated roast beef samples were higher and more consistent in tryptose-sulfite-cycloserine (TSC) agar with or without added egg yolk than in sulfite-polymyxin-sulfadiazine (SPS) agar, specified in the official first action method. The confirmatory technique utilized in the new method was also found to be more reliable than the technique described in the official first action method. Based on the collaborative results, the new method with TSC agar for enumeration and a modified motility-nitrate medium together with a lactose-gelatin medium for confirmation of C. perfringens has been adopted as official first action to replace 46.049-46.053.
在10个实验室开展了一项合作研究，以评估一种用于食品中产气荚膜梭菌营养细胞计数的新方法的性能。将新方法获得的结果与官方首次采用方法（46.049 - 46.053）的结果进行比较。对于接种了产气荚膜梭菌的烤牛肉样品，在添加或不添加蛋黄的胰蛋白胨亚硫酸盐环丝氨酸（TSC）琼脂中，4株产气荚膜梭菌菌株的回收率高于官方首次采用方法中规定的亚硫酸盐多粘菌素磺胺嘧啶（SPS）琼脂，且更为一致。还发现新方法中使用的确证技术比官方首次采用方法中描述的技术更可靠。基于合作研究结果，采用含TSC琼脂计数、改良动力硝酸盐培养基以及乳糖明胶培养基确证产气荚膜梭菌的新方法作为官方首次采用方法，以取代46.049 - 46.053。
